Magnolol nanoparticles exhibit improved water solubility and suppress TNF-α-induced VCAM-1 expression in endothelial cells

Chiang Wen Lee, Stephen Chu Sung Hu, Feng Lin Yen, Lee Fen Hsu, I. Ta Lee, Zih Chan Lin, Ming Horng Tsai, Chieh Liang Huang, Chan Jung Liang*, Yao Chang Chiang

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

14 Scopus citations

Abstract

The expression of the adhesion molecule vascular cell adhesion molecule-1 (VCAM-1) on endothelial cells enables the attachment of leukocytes to the endothelium, which may lead to inflammation and the development of atherosclerosis. Magnolol is a major bioactive compound derived from the plant species Magnolia officinalis. In this study, we synthesized a novel nanoparticle formulation of magnolol to improve its water solubility and physicochemical properties, evaluated its effects on TNF-α-induced VCAM-1 expression in endothelial cells, and determined the signal transduction pathways involved. Our findings demonstrated that the magnolol nanoparticle system showed great improvements in physicochemical properties and water solubility owing to a reduction in particle size, transformation from a crystalline to amorphous structure, and the formation of hydrogen bonds with the nanoparticle carriers. In terms of its biological actions, magnolol nanoparticles attenuated TNF-α-induced VCAM-1 protein expression, promoter activity, and mRNA expression in endothelial cells in vitro. This was found to be mediated by the ERK, AKT, and NF-κB signaling pathways. In addition, magnolol nanoparticles inhibited TNF-α-induced leukocyte adhesion to endothelial cells, and suppressed TNF-α-induced VCAM-1 expression in the aortic endothelium of mice. In summary, since magnolol nanoparticles inhibit endothelial VCAM-1 expression and leukocyte adhesion to endothelial cells, this novel drug formulation may be a potentially useful therapeutic formulation to prevent the development of atherosclerosis and inflammatory diseases.

Original languageEnglish
Pages (from-to)255-268
Number of pages14
JournalJournal of Biomedical Nanotechnology
Volume13
Issue number3
DOIs
StatePublished - 03 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017 American Scientific Publishers All rights reserved.

Keywords

  • Atherosclerosis
  • Endothelial Cells
  • Inflammation
  • Magnolol
  • Nanoparticles
  • Vascular Cell Adhesion Molecule-1

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