TY - JOUR
T1 - MARCO, an innate activation marker of macrophages, is a class A scavenger receptor for Neisseria meningitidis
AU - Mukhopadhyay, Subhankar
AU - Chen, Yunying
AU - Sankala, Marko
AU - Peiser, Leanne
AU - Pikkarainen, Timo
AU - Kraal, Georg
AU - Tryggvason, Karl
AU - Gordon, Siamon
PY - 2006/4
Y1 - 2006/4
N2 - The scavenger receptor-A I/II (SR-A) and macrophage receptor with collagenous domain (MARCO) share a common domain organisation and ligand repertoire, including selected polyanions and gram-positive and -negative organisms, but differ in fine specificity of ligand binding, tissue distribution and regulation. Neisseria meningitidis (NM) is a selective ligand for SR-A, but there is evidence for an additional SR-A-independent, polyanion-sensitive component for NM recognition. We therefore studied the relative contribution of MARCO and SR-A to binding of NM by resident and elicited peritoneal macrophages obtained from MARCO-/-, SR-A-/- and SR-A-MARCO-/- mice. Results confirmed that both mouse and human MARCO are able to bind NM independently of NM LPS. MARCO and SR-A contributed independently to NM binding, correlating with their expression levels in different cell populations, but neither of these two molecules was required for release of TNF-α and nitric oxide. We propose that the TLR-dependent induction of MARCO by innate immune stimulation enhances recognition and uptake of pathogenic organisms such as NM, thus contributing to host defence against infection.
AB - The scavenger receptor-A I/II (SR-A) and macrophage receptor with collagenous domain (MARCO) share a common domain organisation and ligand repertoire, including selected polyanions and gram-positive and -negative organisms, but differ in fine specificity of ligand binding, tissue distribution and regulation. Neisseria meningitidis (NM) is a selective ligand for SR-A, but there is evidence for an additional SR-A-independent, polyanion-sensitive component for NM recognition. We therefore studied the relative contribution of MARCO and SR-A to binding of NM by resident and elicited peritoneal macrophages obtained from MARCO-/-, SR-A-/- and SR-A-MARCO-/- mice. Results confirmed that both mouse and human MARCO are able to bind NM independently of NM LPS. MARCO and SR-A contributed independently to NM binding, correlating with their expression levels in different cell populations, but neither of these two molecules was required for release of TNF-α and nitric oxide. We propose that the TLR-dependent induction of MARCO by innate immune stimulation enhances recognition and uptake of pathogenic organisms such as NM, thus contributing to host defence against infection.
KW - Bacterial infection
KW - Innate immunity
KW - Macrophage
KW - Phagocytosis
UR - http://www.scopus.com/inward/record.url?scp=33645902894&partnerID=8YFLogxK
U2 - 10.1002/eji.200535389
DO - 10.1002/eji.200535389
M3 - 文章
C2 - 16525990
AN - SCOPUS:33645902894
SN - 0014-2980
VL - 36
SP - 940
EP - 949
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 4
ER -