Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (Sensu Stricto)

Shih Hua Teng; Chung Ming Chen; Meng Rui Lee; Tai Fen Lee; Kun Yi Chien; Lee Jene Teng; Po Ren Hsueh

doi:10.1128/JCM.01239-13

Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (Sensu Stricto)

Shih Hua Teng
, Chung Ming Chen
, Meng Rui Lee
, Tai Fen Lee
, Kun Yi Chien
, Lee Jene Teng
, Po Ren Hsueh^*

^*Corresponding author for this work

Department of Biochemistry

Chang Gung University
Bruker Taiwan Co., Ltd.
National Taiwan University
Tao-Yuan General Hospital

Research output: Contribution to journal › Journal Article › peer-review

47 Scopus citations

Abstract

Among 36 Mycobacterium masilliense and 22 M. abscessus isolates identified by erm(41) PCR and sequencing analysis of rpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Original language	English
Pages (from-to)	3113-3116
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	9
DOIs	https://doi.org/10.1128/JCM.01239-13
State	Published - 09 2013

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Teng, S. H., Chen, C. M., Lee, M. R., Lee, T. F., Chien, K. Y., Teng, L. J., & Hsueh, P. R. (2013). Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (Sensu Stricto). Journal of Clinical Microbiology, 51(9), 3113-3116. https://doi.org/10.1128/JCM.01239-13

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title = "Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (Sensu Stricto)",

abstract = "Among 36 Mycobacterium masilliense and 22 M. abscessus isolates identified by erm(41) PCR and sequencing analysis of rpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100\% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.",

author = "Teng, \{Shih Hua\} and Chen, \{Chung Ming\} and Lee, \{Meng Rui\} and Lee, \{Tai Fen\} and Chien, \{Kun Yi\} and Teng, \{Lee Jene\} and Hsueh, \{Po Ren\}",

year = "2013",

month = sep,

doi = "10.1128/JCM.01239-13",

language = "英语",

volume = "51",

pages = "3113--3116",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "9",

}

Teng, SH, Chen, CM, Lee, MR, Lee, TF, Chien, KY, Teng, LJ & Hsueh, PR 2013, 'Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (Sensu Stricto)', Journal of Clinical Microbiology, vol. 51, no. 9, pp. 3113-3116. https://doi.org/10.1128/JCM.01239-13

Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (Sensu Stricto). / Teng, Shih Hua; Chen, Chung Ming; Lee, Meng Rui et al.
In: Journal of Clinical Microbiology, Vol. 51, No. 9, 09.2013, p. 3113-3116.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (Sensu Stricto)

AU - Teng, Shih Hua

AU - Chen, Chung Ming

AU - Lee, Meng Rui

AU - Lee, Tai Fen

AU - Chien, Kun Yi

AU - Teng, Lee Jene

AU - Hsueh, Po Ren

PY - 2013/9

Y1 - 2013/9

N2 - Among 36 Mycobacterium masilliense and 22 M. abscessus isolates identified by erm(41) PCR and sequencing analysis of rpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.

AB - Among 36 Mycobacterium masilliense and 22 M. abscessus isolates identified by erm(41) PCR and sequencing analysis of rpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry.

UR - https://www.scopus.com/pages/publications/84882778720

U2 - 10.1128/JCM.01239-13

DO - 10.1128/JCM.01239-13

M3 - 文章

C2 - 23824775

AN - SCOPUS:84882778720

SN - 0095-1137

VL - 51

SP - 3113

EP - 3116

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 9

ER -

Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (Sensu Stricto)

Abstract

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