Matrix-assisted laser desorption ionization/time-of-flight mass spectrometry for clinical diagnosis

  • Yi Tzu Cho
  • , Hung Su
  • , Tiao Lai Huang
  • , Hung Chun Chen
  • , Wen Jeng Wu
  • , Pei Chang Wu
  • , Deng Chyang Wu
  • , Jentaie Shiea*
  • *Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

46 Scopus citations

Abstract

Background: Matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry is known as an extremely sensitive analytical tool for characterizing different types of biological compounds including proteins, peptides and lipids. Since MALDI-TOF analysis requires very simple sample pretreatment, the technique can be used for rapidly detecting biochemical compounds serving as disease biomarkers. Results: This mini-review focuses on the applications of MALDI-TOF in the detection of potential disease biomarkers in various biological samples. Conclusions: The potential disease biomarkers are mostly abundant proteins, peptides, or lipids including: albumin; hemoglobin; α-defensins; trimethylamine; phospholipids; and glycatedα- and β-globin, which are indicators of albuminuria; fecal occult blood and ischemic stroke; dry eye disease and/or aging; trimethylaniuria; breast cancer; and diabetes, respectively.

Original languageEnglish
Pages (from-to)266-275
Number of pages10
JournalClinica Chimica Acta
Volume415
DOIs
StatePublished - 16 01 2013

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • Clinical diagnosis
  • Disease biomarkers
  • Matrix-assisted laser desorption ionization/time-of-flight mass spectrometry
  • Multivariate analysis
  • Principle component analysis

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