Measuring of binding kinetics of biomolecular interactions using a localized surface plasmon couple fluorescence fiber-optic biosensor

Ying Feng Chang*, Jo Ping Hsieh, Li Chen Su, Ying Chang Li, Cheng Chung Lee, Chien Chou

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

In this study, we describe a novel method for analyzing protein-protein binding kinetics at ultra-low concentration (1 pg/mL) using a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB). The association and dissociation rate constants, ka and kd, respectively, for the binding kinetics of the mouse IgG/ anti-mouse IgG interaction have been calculated to be ka = (5.9928±3.1540)×106 M-1s-1 and kd = (1.0587±0.5572)×10-3 s-1. The theoretical basis of this analytical approach is a rapid-mixing model integrated with a two-compartment model; has been experimentally verified in this study as well. The LSPCF-FOB provides a potentially alternative option for characterizing the interaction of biomolecules at ultra-low concentrations.

Original languageEnglish
Title of host publicationBiosensing III
DOIs
StatePublished - 2010
EventBiosensing III - San Diego, CA, United States
Duration: 01 08 201003 08 2010

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7759
ISSN (Print)0277-786X

Conference

ConferenceBiosensing III
Country/TerritoryUnited States
CitySan Diego, CA
Period01/08/1003/08/10

Keywords

  • Binding kinetics
  • Fiber-optic biosensor
  • Localized surface plasmon

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