Mechanical-Scanned Low-Frequency (28-kHz) Ultrasound to Induce localized Blood-Brain Barrier Disruption

C. Y. Ting, C. H. Pan, H. L. Liu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Recent technical advances showed that focused ultrasound can induce localized blood-brain barrier disruption, however, usually accompanied with the occurrence of hemorrhage. Low-frequency ultrasound has the nature of not been obstructed by skull and has been widely in the industrial applications, but yet have not been applied for biomedical purpose due to its difficulty in concentrating the ultrasonic energy. The purpose of this study is to first demonstrate the feasibility to employ mechanical-scanned low-frequency (28 kHz) ultrasound in localized BBB disruption, which is believed to be the lowest frequency been applied for this application. To examine this, SD rats were anesthesia IV, and ultrasound contrast agent was administered before ultrasound energy excitation to enhance the BBB disruption effect. Blue dye was also administered before animal sacrifice for gross observation of the affected region, and H&E as well as TUNEL staining were performed to evaluate the ultrasound induced brain tissue damage. Results confirmed that low-frequency can be mechanical-scanned for localized BBB-disruption.

Original languageEnglish
Title of host publication13th International Conference on Biomedical Engineering - ICBME 2008
Pages532-535
Number of pages4
DOIs
StatePublished - 2009
Event13th International Conference on Biomedical Engineering, ICBME 2008 - , Singapore
Duration: 03 12 200806 12 2008

Publication series

NameIFMBE Proceedings
Volume23
ISSN (Print)1680-0737

Conference

Conference13th International Conference on Biomedical Engineering, ICBME 2008
Country/TerritorySingapore
Period03/12/0806/12/08

Keywords

  • blood-brain barrier(BBB)
  • low-frequency ultrasound
  • mechanical scan

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