Mechanical stimulation effect on proliferation of murine osteoblast cell

Bo Wun Huang, Feng Sheng Wang, Jih Yang Ko, Wun Han Jhong, Ke Tien Yen, Jung Ge Tseng

Research output: Contribution to journalJournal Article peer-review

4 Scopus citations

Abstract

Age-related diseases, such as osteoporosis, arthritis, accidental fracture, etc., are increased dramatically due to rapid progressing modern medical science and technology push the early arrival of aging society of mankind. How to increase osteoid of the healthy cell, restrain the malignant cell, and fast recovery from bone fracture are becoming major research subjects in the medical community. The growth of bones is proved in medical research to have some relationship with external strength, such as direct current, electromagnetic field, coupled electrical field, ultrasound, etc., and all such researches have their effectiveness in different level. However, few people study the mechanical excitation (vibrating shaker) onto the cell directly in subsonic frequency range. This research studies the culture of mice MC3T3 osteoblast cell in vitro, stimulate the growing cell with mechanical broad range subsonic frequency with or without temperature factor and investigate the effect of different amplitude, repeated number of times, and excitation durations of the stimulation. The cell concentration are then measured by MTT assay by fluorescence spectrometer and RNA assay by electrophoresis diagram and compared with the control (nature growing cell) set. Comparison of different parameters are obtained together with mechanical setup are ready to provide the information about the proliferation of osteoblast for medical community reference.

Original languageEnglish
Pages (from-to)62-67
Number of pages6
JournalLife Science Journal
Volume7
Issue number1
StatePublished - 15 03 2010
Externally publishedYes

Keywords

  • Broadband frequency
  • MTT assay
  • Mechanical stimulation
  • Osteoblast cell
  • RNA assay

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