Metabolism of d-xylose in Schizosaccharomyces pombe cloned with a xylose isomerase gene

Err Cheng Chan*, Peter Pear Ueng, Li Fu Chen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

13 Scopus citations

Abstract

The Escherichia coli xylose isomerase gene was transformed into Schizosaccharomyces pombe for direct d-xylose utilization. In order to understand d-xylose metabolism and determine the limiting factors on d-xylose utilization by the transformed yeast, d-xylose transport, xylose isomerization, and xylulose phosphorylation were investigated. The results indicated that low activity of xylose isomerization in the cloned yeast was the limiting step for d-xylose fermentation. An in vitro study showed that yeast proteases decreased xylose isomerase activity. Xylitol, a by-product of d-xylose fermentation, had no effect on the activity of xylose isomerase.

Original languageEnglish
Pages (from-to)524-528
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume31
Issue number5-6
DOIs
StatePublished - 10 1989
Externally publishedYes

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