Abstract
The Escherichia coli xylose isomerase gene was transformed into Schizosaccharomyces pombe for direct d-xylose utilization. In order to understand d-xylose metabolism and determine the limiting factors on d-xylose utilization by the transformed yeast, d-xylose transport, xylose isomerization, and xylulose phosphorylation were investigated. The results indicated that low activity of xylose isomerization in the cloned yeast was the limiting step for d-xylose fermentation. An in vitro study showed that yeast proteases decreased xylose isomerase activity. Xylitol, a by-product of d-xylose fermentation, had no effect on the activity of xylose isomerase.
| Original language | English |
|---|---|
| Pages (from-to) | 524-528 |
| Number of pages | 5 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 31 |
| Issue number | 5-6 |
| DOIs | |
| State | Published - 10 1989 |
| Externally published | Yes |