Microbial-driven preterm labour involves crosstalk between the innate and adaptive immune response

Denise Chan; Phillip R. Bennett; Yun S. Lee; Samit Kundu; T. G. Teoh; Malko Adan; Saqa Ahmed; Richard G. Brown; Anna L. David; Holly V. Lewis; Belen Gimeno-Molina; Jane E. Norman; Sarah J. Stock; Vasso Terzidou; Pascale Kropf; Marina Botto; David A. MacIntyre; Lynne Sykes

doi:10.1038/s41467-022-28620-1

Microbial-driven preterm labour involves crosstalk between the innate and adaptive immune response

Denise Chan, Phillip R. Bennett, Yun S. Lee, Samit Kundu, T. G. Teoh, Malko Adan, Saqa Ahmed, Richard G. Brown, Anna L. David, Holly V. Lewis, Belen Gimeno-Molina, Jane E. Norman, Sarah J. Stock, Vasso Terzidou, Pascale Kropf, Marina Botto, David A. MacIntyre, Lynne Sykes^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

49 Scopus citations

Abstract

There has been a surge in studies implicating a role of vaginal microbiota in spontaneous preterm birth (sPTB), but most are associative without mechanistic insight. Here we show a comprehensive approach to understand the causative factors of preterm birth, based on the integration of longitudinal vaginal microbiota and cervicovaginal fluid (CVF) immunophenotype data collected from 133 women at high-risk of sPTB. We show that vaginal depletion of Lactobacillus species and high bacterial diversity leads to increased mannose binding lectin (MBL), IgM, IgG, C3b, C5, IL-8, IL-6 and IL-1β and to increased risk of sPTB. Cervical shortening, which often precedes preterm birth, is associated with Lactobacillus iners and elevated levels of IgM, C3b, C5, C5a and IL-6. These data demonstrate a role for the complement system in microbial-driven sPTB and provide a scientific rationale for the development of live biotherapeutics and complement therapeutics to prevent sPTB.

Original language	English
Article number	975
Journal	Nature Communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-28620-1
State	Published - 12 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022, The Author(s).

Access to Document

10.1038/s41467-022-28620-1

Cite this

Chan, D., Bennett, P. R., Lee, Y. S., Kundu, S., Teoh, T. G., Adan, M., Ahmed, S., Brown, R. G., David, A. L., Lewis, H. V., Gimeno-Molina, B., Norman, J. E., Stock, S. J., Terzidou, V., Kropf, P., Botto, M., MacIntyre, D. A., & Sykes, L. (2022). Microbial-driven preterm labour involves crosstalk between the innate and adaptive immune response. Nature Communications, 13(1), Article 975. https://doi.org/10.1038/s41467-022-28620-1

@article{8d48f8048a504d6bbacf4d0b24da1a2d,

title = "Microbial-driven preterm labour involves crosstalk between the innate and adaptive immune response",

abstract = "There has been a surge in studies implicating a role of vaginal microbiota in spontaneous preterm birth (sPTB), but most are associative without mechanistic insight. Here we show a comprehensive approach to understand the causative factors of preterm birth, based on the integration of longitudinal vaginal microbiota and cervicovaginal fluid (CVF) immunophenotype data collected from 133 women at high-risk of sPTB. We show that vaginal depletion of Lactobacillus species and high bacterial diversity leads to increased mannose binding lectin (MBL), IgM, IgG, C3b, C5, IL-8, IL-6 and IL-1β and to increased risk of sPTB. Cervical shortening, which often precedes preterm birth, is associated with Lactobacillus iners and elevated levels of IgM, C3b, C5, C5a and IL-6. These data demonstrate a role for the complement system in microbial-driven sPTB and provide a scientific rationale for the development of live biotherapeutics and complement therapeutics to prevent sPTB.",

author = "Denise Chan and Bennett, {Phillip R.} and Lee, {Yun S.} and Samit Kundu and Teoh, {T. G.} and Malko Adan and Saqa Ahmed and Brown, {Richard G.} and David, {Anna L.} and Lewis, {Holly V.} and Belen Gimeno-Molina and Norman, {Jane E.} and Stock, {Sarah J.} and Vasso Terzidou and Pascale Kropf and Marina Botto and MacIntyre, {David A.} and Lynne Sykes",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1038/s41467-022-28620-1",

language = "英语",

volume = "13",

journal = "Nature Communications",

issn = "2041-1723",

number = "1",

}

Chan, D, Bennett, PR, Lee, YS, Kundu, S, Teoh, TG, Adan, M, Ahmed, S, Brown, RG, David, AL, Lewis, HV, Gimeno-Molina, B, Norman, JE, Stock, SJ, Terzidou, V, Kropf, P, Botto, M, MacIntyre, DA & Sykes, L 2022, 'Microbial-driven preterm labour involves crosstalk between the innate and adaptive immune response', Nature Communications, vol. 13, no. 1, 975. https://doi.org/10.1038/s41467-022-28620-1

TY - JOUR

T1 - Microbial-driven preterm labour involves crosstalk between the innate and adaptive immune response

AU - Chan, Denise

AU - Bennett, Phillip R.

AU - Lee, Yun S.

AU - Kundu, Samit

AU - Teoh, T. G.

AU - Adan, Malko

AU - Ahmed, Saqa

AU - Brown, Richard G.

AU - David, Anna L.

AU - Lewis, Holly V.

AU - Gimeno-Molina, Belen

AU - Norman, Jane E.

AU - Stock, Sarah J.

AU - Terzidou, Vasso

AU - Kropf, Pascale

AU - Botto, Marina

AU - MacIntyre, David A.

AU - Sykes, Lynne

PY - 2022/12

Y1 - 2022/12

N2 - There has been a surge in studies implicating a role of vaginal microbiota in spontaneous preterm birth (sPTB), but most are associative without mechanistic insight. Here we show a comprehensive approach to understand the causative factors of preterm birth, based on the integration of longitudinal vaginal microbiota and cervicovaginal fluid (CVF) immunophenotype data collected from 133 women at high-risk of sPTB. We show that vaginal depletion of Lactobacillus species and high bacterial diversity leads to increased mannose binding lectin (MBL), IgM, IgG, C3b, C5, IL-8, IL-6 and IL-1β and to increased risk of sPTB. Cervical shortening, which often precedes preterm birth, is associated with Lactobacillus iners and elevated levels of IgM, C3b, C5, C5a and IL-6. These data demonstrate a role for the complement system in microbial-driven sPTB and provide a scientific rationale for the development of live biotherapeutics and complement therapeutics to prevent sPTB.

AB - There has been a surge in studies implicating a role of vaginal microbiota in spontaneous preterm birth (sPTB), but most are associative without mechanistic insight. Here we show a comprehensive approach to understand the causative factors of preterm birth, based on the integration of longitudinal vaginal microbiota and cervicovaginal fluid (CVF) immunophenotype data collected from 133 women at high-risk of sPTB. We show that vaginal depletion of Lactobacillus species and high bacterial diversity leads to increased mannose binding lectin (MBL), IgM, IgG, C3b, C5, IL-8, IL-6 and IL-1β and to increased risk of sPTB. Cervical shortening, which often precedes preterm birth, is associated with Lactobacillus iners and elevated levels of IgM, C3b, C5, C5a and IL-6. These data demonstrate a role for the complement system in microbial-driven sPTB and provide a scientific rationale for the development of live biotherapeutics and complement therapeutics to prevent sPTB.

UR - http://www.scopus.com/inward/record.url?scp=85125154824&partnerID=8YFLogxK

U2 - 10.1038/s41467-022-28620-1

DO - 10.1038/s41467-022-28620-1

M3 - 文章

C2 - 35190561

AN - SCOPUS:85125154824

SN - 2041-1723

VL - 13

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 975

ER -

Microbial-driven preterm labour involves crosstalk between the innate and adaptive immune response

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this