Microbiological degradation of phenol using mixed liquors of Pseudomonas putida and activated sludge

Gurusamy Annadurai, Ruey Shin Juang*, Duu Jong Lee

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

97 Scopus citations

Abstract

This work investigated the biodegradation potential of phenol using mixed liquors of Pseudomonas putida (ATCC 31800) and activated sludge. Experiments were made as a function of solution pH (6-10), temperature (30-36 °C), nitrogen source (NH4)2SO4 (0.5-0.8 g/l), and carbon source glucose (0.5-0.8 g/l). Response surface methodology by the Box-Behnken model was used to examine the role of four process factors on phenol degradation. It was shown that a second-order polynomial regression model could properly interpret the experimental data with an R2-value of 0.9997 and an F-value of 3605.45, based on which the maximum degradation of phenol was estimated up to 80.1% within the range examined. Interactions between process parameters and each significance effect on phenol degradation were also discussed.

Original languageEnglish
Pages (from-to)703-710
Number of pages8
JournalWaste Management
Volume22
Issue number7
DOIs
StatePublished - 11 2002
Externally publishedYes

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