TY - GEN
T1 - Microdfluidic based 3-dimensional cell culture platform
AU - Huang, Song Bin
AU - Wu, Min Hsien
AU - Cui, Zhanfeng
AU - Cui, Zheng
AU - Lee, Gwo Bin
PY - 2008
Y1 - 2008
N2 - This study reports a new perfusion-based, micro threedimensional (3-D) cell culture platform for drug testing using enabling microfluidic technologies. In this work, a perfusionbased, micro 3-D cell culture platform is designed and is fabricated based on SU-8 lithography and polydimethylsiloxane (PDMS) replication processes. One of the key features of the system is that the incorporation of a multiple medium pumping mechanism, consisting of 15 membranebased pneumatic micropumps with serpentine-shape (S-shape) layout, coupled with a pneumatic tank, into the micro 3-D cell culture platform to provide efficient and economical culture medium delivery. Moreover, a "smart cell/agarose (scaffold) loading mechanism" was proposed, allowing the cell/3-D scaffold loading process in one step and avoiding too much laborious works and manual error. The results show that in all of the 15 S-shape pneumatic micropumps studied, the medium delivery mechanism is able to provide a uniform flow output ranging from 5.5 to 131 μl/hr depending on the applied pulsation frequency of the micropumps. In addition, the cell/agarose (scaffold) loading mechanism was proved to be able to perform sample loading tasks precisely and accurately in all of the 15 microbioreactors integrated. Furthermore, anticancer drug testing was successfully demonstrated using the proposed culture platform and fluorescent microscopic observation. As a whole, because of miniaturization, not only does this perfusion 3-D cell culture platform provide a homogenous and steady cell culture environment, but it also reduces the need for human intervention. Moreover, due to the integrated pumping of the medium and the cell/agarose (scaffold) loading mechanisms, time efficient and economical research work can be achieved. These characteristics are found particularly useful for high-precision and high-throughput 3-D cell culture-based drug testing.
AB - This study reports a new perfusion-based, micro threedimensional (3-D) cell culture platform for drug testing using enabling microfluidic technologies. In this work, a perfusionbased, micro 3-D cell culture platform is designed and is fabricated based on SU-8 lithography and polydimethylsiloxane (PDMS) replication processes. One of the key features of the system is that the incorporation of a multiple medium pumping mechanism, consisting of 15 membranebased pneumatic micropumps with serpentine-shape (S-shape) layout, coupled with a pneumatic tank, into the micro 3-D cell culture platform to provide efficient and economical culture medium delivery. Moreover, a "smart cell/agarose (scaffold) loading mechanism" was proposed, allowing the cell/3-D scaffold loading process in one step and avoiding too much laborious works and manual error. The results show that in all of the 15 S-shape pneumatic micropumps studied, the medium delivery mechanism is able to provide a uniform flow output ranging from 5.5 to 131 μl/hr depending on the applied pulsation frequency of the micropumps. In addition, the cell/agarose (scaffold) loading mechanism was proved to be able to perform sample loading tasks precisely and accurately in all of the 15 microbioreactors integrated. Furthermore, anticancer drug testing was successfully demonstrated using the proposed culture platform and fluorescent microscopic observation. As a whole, because of miniaturization, not only does this perfusion 3-D cell culture platform provide a homogenous and steady cell culture environment, but it also reduces the need for human intervention. Moreover, due to the integrated pumping of the medium and the cell/agarose (scaffold) loading mechanisms, time efficient and economical research work can be achieved. These characteristics are found particularly useful for high-precision and high-throughput 3-D cell culture-based drug testing.
KW - 3-D cell culture
KW - Drug testing
KW - Microbioreactors
KW - Microfluidic devices
KW - Perfusion cell culture
UR - http://www.scopus.com/inward/record.url?scp=49449093289&partnerID=8YFLogxK
U2 - 10.1115/MNHT2008-52292
DO - 10.1115/MNHT2008-52292
M3 - 会议稿件
AN - SCOPUS:49449093289
SN - 0791842924
SN - 9780791842928
T3 - 2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008
SP - 279
EP - 284
BT - 2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008
T2 - 1st ASME Micro/Nanoscale Heat Transfer International Conference, MNHT08
Y2 - 6 January 2008 through 9 January 2008
ER -