Microelectrode array biosensor for the detection of Legionella pneumophila

Kin Fong Lei; Polly H.M. Leung

doi:10.1016/j.mee.2011.10.002

Microelectrode array biosensor for the detection of Legionella pneumophila

Kin Fong Lei^*, Polly H.M. Leung

^*Corresponding author for this work

Master Program in Nano-Electronic Engineering and Design

Hong Kong Polytechnic University

Research output: Contribution to journal › Journal Article › peer-review

29 Scopus citations

Abstract

A microelectrode array biosensor has been developed for the detection of Legionella pneumophila serogroup 5 suspended in solution. The biosensor consists of a glass substrate with measuring microelectrodes and a PDMS layer providing a reservoir on the glass surface. The surface of the microelectrodes is functionalized and the analyte-specific antibodies are immobilized to form a biological transducer. The detection is based on measuring the impedance changes across the electrodes which is immersed in the bacteria suspension contained in the reservoir. Therefore, the bacterial concentration in solution can be estimated by impedance change. It is feasible to detect Legionella concentration from 10⁵ to 10⁸ colony-forming units (CFU)/ml. Since this method provides a fast and easy measurement, it has the potential to be developed into miniaturized equipment that can be installed in cooling towers for the continuous monitoring of Legionella concentration.

Original language	English
Pages (from-to)	174-177
Number of pages	4
Journal	Microelectronic Engineering
Volume	91
DOIs	https://doi.org/10.1016/j.mee.2011.10.002
State	Published - 03 2012

Keywords

Biosensor
Impedance measurement
Legionnaires' disease
Microfluidics

Access to Document

10.1016/j.mee.2011.10.002

Cite this

@article{1353115cd7ae4120a8c4d6e976923a42,

title = "Microelectrode array biosensor for the detection of Legionella pneumophila",

abstract = "A microelectrode array biosensor has been developed for the detection of Legionella pneumophila serogroup 5 suspended in solution. The biosensor consists of a glass substrate with measuring microelectrodes and a PDMS layer providing a reservoir on the glass surface. The surface of the microelectrodes is functionalized and the analyte-specific antibodies are immobilized to form a biological transducer. The detection is based on measuring the impedance changes across the electrodes which is immersed in the bacteria suspension contained in the reservoir. Therefore, the bacterial concentration in solution can be estimated by impedance change. It is feasible to detect Legionella concentration from 105 to 108 colony-forming units (CFU)/ml. Since this method provides a fast and easy measurement, it has the potential to be developed into miniaturized equipment that can be installed in cooling towers for the continuous monitoring of Legionella concentration.",

keywords = "Biosensor, Impedance measurement, Legionnaires' disease, Microfluidics",

author = "Lei, {Kin Fong} and Leung, {Polly H.M.}",

year = "2012",

month = mar,

doi = "10.1016/j.mee.2011.10.002",

language = "英语",

volume = "91",

pages = "174--177",

journal = "Microelectronic Engineering",

issn = "0167-9317",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Microelectrode array biosensor for the detection of Legionella pneumophila

AU - Lei, Kin Fong

AU - Leung, Polly H.M.

PY - 2012/3

Y1 - 2012/3

N2 - A microelectrode array biosensor has been developed for the detection of Legionella pneumophila serogroup 5 suspended in solution. The biosensor consists of a glass substrate with measuring microelectrodes and a PDMS layer providing a reservoir on the glass surface. The surface of the microelectrodes is functionalized and the analyte-specific antibodies are immobilized to form a biological transducer. The detection is based on measuring the impedance changes across the electrodes which is immersed in the bacteria suspension contained in the reservoir. Therefore, the bacterial concentration in solution can be estimated by impedance change. It is feasible to detect Legionella concentration from 105 to 108 colony-forming units (CFU)/ml. Since this method provides a fast and easy measurement, it has the potential to be developed into miniaturized equipment that can be installed in cooling towers for the continuous monitoring of Legionella concentration.

AB - A microelectrode array biosensor has been developed for the detection of Legionella pneumophila serogroup 5 suspended in solution. The biosensor consists of a glass substrate with measuring microelectrodes and a PDMS layer providing a reservoir on the glass surface. The surface of the microelectrodes is functionalized and the analyte-specific antibodies are immobilized to form a biological transducer. The detection is based on measuring the impedance changes across the electrodes which is immersed in the bacteria suspension contained in the reservoir. Therefore, the bacterial concentration in solution can be estimated by impedance change. It is feasible to detect Legionella concentration from 105 to 108 colony-forming units (CFU)/ml. Since this method provides a fast and easy measurement, it has the potential to be developed into miniaturized equipment that can be installed in cooling towers for the continuous monitoring of Legionella concentration.

KW - Biosensor

KW - Impedance measurement

KW - Legionnaires' disease

KW - Microfluidics

UR - http://www.scopus.com/inward/record.url?scp=84855552840&partnerID=8YFLogxK

U2 - 10.1016/j.mee.2011.10.002

DO - 10.1016/j.mee.2011.10.002

M3 - 文章

AN - SCOPUS:84855552840

SN - 0167-9317

VL - 91

SP - 174

EP - 177

JO - Microelectronic Engineering

JF - Microelectronic Engineering

ER -

Microelectrode array biosensor for the detection of Legionella pneumophila

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this