Microfluidic chip of immunoassay system for kidney studies

Bo Jyun Zhang; Chung Wei Huang; Kuo Wei Chang; Chih Wei Yang; Cheng Chieh Hung; Yi Ching Ko; Li Fang Chou; Gaurav Pendharkar; Cheng Hsien Liu

doi:10.1109/TRANSDUCERS.2017.7994405

Microfluidic chip of immunoassay system for kidney studies

Bo Jyun Zhang^*, Chung Wei Huang, Kuo Wei Chang, Chih Wei Yang, Cheng Chieh Hung, Yi Ching Ko, Li Fang Chou, Gaurav Pendharkar, Cheng Hsien Liu

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

In this research, we have demonstrated a microfluidic chip which is able to manipulate and pattern the cell in the different culture chamber for Kidney studies integrated the dielectrophoresis (DEP) force. This design enables Escherichia coli (E. coli) Lipopolysaccharides (LPS) to stimulate human monocytic leukemia cell line (THP-1) without contact proximal tubular cell (HK-2). Therefore, cell-cell interaction can only be achieved by the transmission of cytokine. Epithelial-mesenchymal transition (EMT) of HK-2 was observed after treated with IL-1β 150pg/ml for 72 hours in a 24-well plate. On the other hand, the result of DEP patterning shows a potential way to make cells proliferate on the condition of adjustable and appropriate cell density.

Original language	English
Title of host publication	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1746-1749
Number of pages	4
ISBN (Electronic)	9781538627310
DOIs	https://doi.org/10.1109/TRANSDUCERS.2017.7994405
State	Published - 26 07 2017
Externally published	Yes
Event	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan Duration: 18 06 2017 → 22 06 2017

Publication series

Name	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

Conference	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Country/Territory	Taiwan
City	Kaohsiung
Period	18/06/17 → 22/06/17

Bibliographical note

Publisher Copyright:
© 2017 IEEE.

Keywords

Cell-Cell Interaction
Dielectrophoresis
Immune Response
Kidney
Microfluidic

Access to Document

10.1109/TRANSDUCERS.2017.7994405

Cite this

Zhang, B. J., Huang, C. W., Chang, K. W., Yang, C. W., Hung, C. C., Ko, Y. C., Chou, L. F., Pendharkar, G., & Liu, C. H. (2017). Microfluidic chip of immunoassay system for kidney studies. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 1746-1749). Article 7994405 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994405

Zhang, Bo Jyun ; Huang, Chung Wei ; Chang, Kuo Wei et al. / Microfluidic chip of immunoassay system for kidney studies. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 1746-1749 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

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title = "Microfluidic chip of immunoassay system for kidney studies",

abstract = "In this research, we have demonstrated a microfluidic chip which is able to manipulate and pattern the cell in the different culture chamber for Kidney studies integrated the dielectrophoresis (DEP) force. This design enables Escherichia coli (E. coli) Lipopolysaccharides (LPS) to stimulate human monocytic leukemia cell line (THP-1) without contact proximal tubular cell (HK-2). Therefore, cell-cell interaction can only be achieved by the transmission of cytokine. Epithelial-mesenchymal transition (EMT) of HK-2 was observed after treated with IL-1β 150pg/ml for 72 hours in a 24-well plate. On the other hand, the result of DEP patterning shows a potential way to make cells proliferate on the condition of adjustable and appropriate cell density.",

keywords = "Cell-Cell Interaction, Dielectrophoresis, Immune Response, Kidney, Microfluidic",

author = "Zhang, {Bo Jyun} and Huang, {Chung Wei} and Chang, {Kuo Wei} and Yang, {Chih Wei} and Hung, {Cheng Chieh} and Ko, {Yi Ching} and Chou, {Li Fang} and Gaurav Pendharkar and Liu, {Cheng Hsien}",

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year = "2017",

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day = "26",

doi = "10.1109/TRANSDUCERS.2017.7994405",

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series = "TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems",

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Zhang, BJ, Huang, CW, Chang, KW, Yang, CW, Hung, CC, Ko, YC, Chou, LF, Pendharkar, G & Liu, CH 2017, Microfluidic chip of immunoassay system for kidney studies. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994405, TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, Institute of Electrical and Electronics Engineers Inc., pp. 1746-1749, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, 18/06/17. https://doi.org/10.1109/TRANSDUCERS.2017.7994405

Microfluidic chip of immunoassay system for kidney studies. / Zhang, Bo Jyun; Huang, Chung Wei; Chang, Kuo Wei et al.
TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 1746-1749 7994405 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Microfluidic chip of immunoassay system for kidney studies

AU - Zhang, Bo Jyun

AU - Huang, Chung Wei

AU - Chang, Kuo Wei

AU - Yang, Chih Wei

AU - Hung, Cheng Chieh

AU - Ko, Yi Ching

AU - Chou, Li Fang

AU - Pendharkar, Gaurav

AU - Liu, Cheng Hsien

PY - 2017/7/26

Y1 - 2017/7/26

N2 - In this research, we have demonstrated a microfluidic chip which is able to manipulate and pattern the cell in the different culture chamber for Kidney studies integrated the dielectrophoresis (DEP) force. This design enables Escherichia coli (E. coli) Lipopolysaccharides (LPS) to stimulate human monocytic leukemia cell line (THP-1) without contact proximal tubular cell (HK-2). Therefore, cell-cell interaction can only be achieved by the transmission of cytokine. Epithelial-mesenchymal transition (EMT) of HK-2 was observed after treated with IL-1β 150pg/ml for 72 hours in a 24-well plate. On the other hand, the result of DEP patterning shows a potential way to make cells proliferate on the condition of adjustable and appropriate cell density.

AB - In this research, we have demonstrated a microfluidic chip which is able to manipulate and pattern the cell in the different culture chamber for Kidney studies integrated the dielectrophoresis (DEP) force. This design enables Escherichia coli (E. coli) Lipopolysaccharides (LPS) to stimulate human monocytic leukemia cell line (THP-1) without contact proximal tubular cell (HK-2). Therefore, cell-cell interaction can only be achieved by the transmission of cytokine. Epithelial-mesenchymal transition (EMT) of HK-2 was observed after treated with IL-1β 150pg/ml for 72 hours in a 24-well plate. On the other hand, the result of DEP patterning shows a potential way to make cells proliferate on the condition of adjustable and appropriate cell density.

KW - Cell-Cell Interaction

KW - Dielectrophoresis

KW - Immune Response

KW - Kidney

KW - Microfluidic

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BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017

Y2 - 18 June 2017 through 22 June 2017

ER -

Zhang BJ, Huang CW, Chang KW, Yang CW, Hung CC, Ko YC et al. Microfluidic chip of immunoassay system for kidney studies. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 1746-1749. 7994405. (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). doi: 10.1109/TRANSDUCERS.2017.7994405

Microfluidic chip of immunoassay system for kidney studies

Abstract

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Bibliographical note

Keywords

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