Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

Lien Yu Hung; Chien Yu Fu; Chih Hung Wang; Yuan Jhe Chuang; Yi Cheng Tsai; Yi Ling Lo; Wen Bin Lee; Shu Chu Shiesh; Hwan You Chang; Keng Fu Hsu; Gwo Bin Lee

doi:10.1109/TRANSDUCERS.2017.7994112

Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

Lien Yu Hung
, Chien Yu Fu
, Chih Hung Wang
, Yuan Jhe Chuang
, Yi Cheng Tsai
, Yi Ling Lo
, Wen Bin Lee
, Shu Chu Shiesh
, Hwan You Chang
, Keng Fu Hsu
, Gwo Bin Lee

National Tsing Hua University
National Cheng Kung University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

Original language	English
Title of host publication	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	568-571
Number of pages	4
ISBN (Electronic)	9781538627310
DOIs	https://doi.org/10.1109/TRANSDUCERS.2017.7994112
State	Published - 26 07 2017
Externally published	Yes
Event	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan Duration: 18 06 2017 → 22 06 2017

Publication series

Name	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

Conference	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Country/Territory	Taiwan
City	Kaohsiung
Period	18/06/17 → 22/06/17

Bibliographical note

Publisher Copyright:
© 2017 IEEE.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Affinity reagents
Aptamers
Ovarian cancer
Peptides
Phage display
SELEX

Access to Document

10.1109/TRANSDUCERS.2017.7994112

Cite this

Hung, L. Y., Fu, C. Y., Wang, C. H., Chuang, Y. J., Tsai, Y. C., Lo, Y. L., Lee, W. B., Shiesh, S. C., Chang, H. Y., Hsu, K. F., & Lee, G. B. (2017). Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 568-571). Article 7994112 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994112

Hung, Lien Yu ; Fu, Chien Yu ; Wang, Chih Hung et al. / Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 568-571 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

@inproceedings{a871e5bdd38f4706b01ce46658f91fb3,

title = "Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer",

abstract = "This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.",

keywords = "Affinity reagents, Aptamers, Ovarian cancer, Peptides, Phage display, SELEX",

author = "Hung, \{Lien Yu\} and Fu, \{Chien Yu\} and Wang, \{Chih Hung\} and Chuang, \{Yuan Jhe\} and Tsai, \{Yi Cheng\} and Lo, \{Yi Ling\} and Lee, \{Wen Bin\} and Shiesh, \{Shu Chu\} and Chang, \{Hwan You\} and Hsu, \{Keng Fu\} and Lee, \{Gwo Bin\}",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 ; Conference date: 18-06-2017 Through 22-06-2017",

year = "2017",

month = jul,

day = "26",

doi = "10.1109/TRANSDUCERS.2017.7994112",

language = "英语",

series = "TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "568--571",

booktitle = "TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems",

address = "美国",

}

Hung, LY, Fu, CY, Wang, CH, Chuang, YJ, Tsai, YC, Lo, YL, Lee, WB, Shiesh, SC, Chang, HY, Hsu, KF & Lee, GB 2017, Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994112, TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, Institute of Electrical and Electronics Engineers Inc., pp. 568-571, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, 18/06/17. https://doi.org/10.1109/TRANSDUCERS.2017.7994112

Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. / Hung, Lien Yu; Fu, Chien Yu; Wang, Chih Hung et al.
TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 568-571 7994112 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

AU - Hung, Lien Yu

AU - Fu, Chien Yu

AU - Wang, Chih Hung

AU - Chuang, Yuan Jhe

AU - Tsai, Yi Cheng

AU - Lo, Yi Ling

AU - Lee, Wen Bin

AU - Shiesh, Shu Chu

AU - Chang, Hwan You

AU - Hsu, Keng Fu

AU - Lee, Gwo Bin

PY - 2017/7/26

Y1 - 2017/7/26

N2 - This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

AB - This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

KW - Affinity reagents

KW - Aptamers

KW - Ovarian cancer

KW - Peptides

KW - Phage display

KW - SELEX

UR - https://www.scopus.com/pages/publications/85029361482

U2 - 10.1109/TRANSDUCERS.2017.7994112

DO - 10.1109/TRANSDUCERS.2017.7994112

M3 - 会议稿件

AN - SCOPUS:85029361482

T3 - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

SP - 568

EP - 571

BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017

Y2 - 18 June 2017 through 22 June 2017

ER -

Hung LY, Fu CY, Wang CH, Chuang YJ, Tsai YC, Lo YL et al. Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 568-571. 7994112. (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). doi: 10.1109/TRANSDUCERS.2017.7994112

Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Keywords

Access to Document

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