Abstract
An ELISA for urine microalbumin using microtiter plates has been developed. The assay uses polyclonal anti-human albumin antibody for coating the microtiter plates and the same antibody conjugated with horseradish peroxidase for detection. The assay sensitivity is 1.6 μg/ml. Results by this in-house ELISA show good correlation (r = 0.99) with those obtained by a commercial assay using the Behring BNII autoanalyzer. Within-day and between-day CVs are 10%. Reference values for microalbumin in 769 urine specimens from healthy Chinese subjects were higher in women than men and higher in subjects 50 yr than in those <50 yr of age. Elevated mean concentrations of urine microalbumin were observed in patients with type 2 diabetes and CVD. This in-house ELISA is simple, sensitive, precise, and especially suited for laboratories without expensive autoanalyzers.
| Original language | English |
|---|---|
| Pages (from-to) | 149-154 |
| Number of pages | 6 |
| Journal | Annals of Clinical and Laboratory Science |
| Volume | 35 |
| Issue number | 2 |
| State | Published - 03 2005 |
| Externally published | Yes |
Keywords
- Cardiovascular disease
- Enzyme-linked immunosorbent assay
- Microtiter plate assay
- Type 2 diabetes
- Urine microalbumin
