Mobile Phase Selectivity in the Separation of Epimeric and Positionally Isomeric Triterpenoids by Reversed Phase High Performance Liquid Chromatography

Ming‐Shi ‐S Shiao*, Lee‐Juian ‐J Lin

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

2 Scopus citations

Abstract

The chromatographic behavior of 8 lanostanoid triterpenes including 4 pairs of C‐3 epimers and 2 pairs of C‐3/C‐15 positional isomers was studied by reversed phase HPLC. A consistent elution sequence of these paired isomers in all combination of three distinct solvent systems was observed. The mobile phase selectivity to separate specific, paired isomers was mainly dependent on the polarity difference contributed by C‐3/C‐15 hydroxyl and acetoxyl groups. The stereochemistry at C‐3 played more profound role in guiding the hydrophobic interaction with C18 stationary phase in the separation of C‐3/C‐15 positional isomers.

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalJournal of the Chinese Chemical Society
Volume36
Issue number5
DOIs
StatePublished - 10 1989
Externally publishedYes

Keywords

  • epimers
  • positional isomers: HPLC
  • triterpenoids

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