Abstract
The chromatographic behavior of 8 lanostanoid triterpenes including 4 pairs of C‐3 epimers and 2 pairs of C‐3/C‐15 positional isomers was studied by reversed phase HPLC. A consistent elution sequence of these paired isomers in all combination of three distinct solvent systems was observed. The mobile phase selectivity to separate specific, paired isomers was mainly dependent on the polarity difference contributed by C‐3/C‐15 hydroxyl and acetoxyl groups. The stereochemistry at C‐3 played more profound role in guiding the hydrophobic interaction with C18 stationary phase in the separation of C‐3/C‐15 positional isomers.
Original language | English |
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Pages (from-to) | 429-434 |
Number of pages | 6 |
Journal | Journal of the Chinese Chemical Society |
Volume | 36 |
Issue number | 5 |
DOIs | |
State | Published - 10 1989 |
Externally published | Yes |
Keywords
- epimers
- positional isomers: HPLC
- triterpenoids