Abstract
Hydroxyl-terminated polybutadiene (HTPB)-based polyurethane with dimethyol propionic acid (DPA) as chain extender was synthesized by solution polymerization. The HTPB-based polyurethane was modified by UV radiation with N-isopropyl acrylamide monomer to get poly(N-isopropyl acrylamide)-modified polyurethane (PUDPANIPAAm). The cohesive energy (Ecoh), molar volume (V), solubility parameter (δ), molecular weight (WM), volume per gram (Vg), and the density (1/Vg) of PUDPANIPAAm were calculated by group contribution methods. To evaluate the application of PUDPANIPAAm for wound dressing and transplantation of cell sheet, the measurement of water content, water vapor transmission rate, and gas permeation on the PUDPANIPAAm membrane was evaluated. The biocompatibility of these membranes, cell adhesion, and proliferation assay were conducted in the cell culture. The effect of thermosensitivity of poly(N-isopropyl acrylamide) on cell detachment was also evaluated in the primary study. The results showed that these PUDPANIPAAm membranes are thermosensitive. The modification of PU with poly(N-isopropyl acrylamide) reduced the water vapor transmission rate and permeability of gas through PUDPANIPAAm membrane. PUDPANIPAAm membranes could support cell adhesion and growth. Owing to the thermosensitive nature of poly(N-isopropyl acrylamide), the relative cell numbers detached from PUDPANIPAAm membranes were larger than those detached from the polystyrene dish.
Original language | English |
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Pages (from-to) | 43-51 |
Number of pages | 9 |
Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
Volume | 80 |
Issue number | 1 |
DOIs | |
State | Published - 01 2007 |
Keywords
- Cell culture
- Poly(N-isopropyl acrylamide)
- Polyurethane
- Thermosensitivity
- Wound dressing