Molecular changes after shockwave therapy in osteoarthritic knee in rats

C. J. Wang; Y. C. Sun; C. T. Wu; L. H. Weng; F. S. Wang

doi:10.1007/s00193-013-0480-5

Molecular changes after shockwave therapy in osteoarthritic knee in rats

C. J. Wang^*, Y. C. Sun, C. T. Wu, L. H. Weng, F. S. Wang

^*Corresponding author for this work

School of Medicine

Chang Gung University

Research output: Contribution to journal › Journal Article › peer-review

4 Scopus citations

Abstract

This study investigated the molecular changes of DKK-1, MMP13, Wnt-5a and β-catenin after extracorporeal shock wave therapy (ESWT) in anterior cruciateligament transected (ACLT) osteoarthritic (OA) knee in rats. 27 male Spraque-Dawley rats were divided into three groups. Group I was the control one and received sham knee arthrotomy but no ACLT or ESWT. Group II underwent ACLT, but no ESWT. Group III underwent ACLT and received ESWT. The animalswerekilledat12weeks,andtheharvestedkneespec- imens were subjected to histo pathological examination and immune histo chemical analysis. Radiographs of the knees were obtained at 0 and 12 weeks. At 12 weeks, radiographs of group II showed more arthritic changes with formation of osteochondral fragments, whereas very subtle arthritis was noted in groups I and III. In histopathological examination, group II showed a significant increase of Mankin score and a decrease of sub chondral bone as compared to groups I and III. Group III showed a significant decrease of Mankin score and an increase of subchondral bone, with the data comparable to group I. In immunohistochemical analysis, group II showed significant increases of DKK-1 and MMP13 and decreases of Wnt-5a and β-catenin in articular cartilage and subchondral bone as compared to groups I and III. Group III showed sig-nificant decreases of DKK-1 and MMP13 and increases of Wnt-5a and β-catenin, with the data comparable to group I. In conclusion, the application of ESWT causes molecular changes that are consistent with the improvement in sub-chondral bone remodeling and chondroprotective effect in ACLT OA knees in rats.

Original language	English
Pages (from-to)	45-51
Number of pages	7
Journal	Shock Waves
Volume	26
Issue number	1
DOIs	https://doi.org/10.1007/s00193-013-0480-5
State	Published - 01 2016

Bibliographical note

Publisher Copyright:
© Springer-Verlag Berlin Heidelberg 2013.

Keywords

ACLT
ESWT
Molecular change
OA knee
Rats

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10.1007/s00193-013-0480-5

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title = "Molecular changes after shockwave therapy in osteoarthritic knee in rats",

abstract = "This study investigated the molecular changes of DKK-1, MMP13, Wnt-5a and β-catenin after extracorporeal shock wave therapy (ESWT) in anterior cruciateligament transected (ACLT) osteoarthritic (OA) knee in rats. 27 male Spraque-Dawley rats were divided into three groups. Group I was the control one and received sham knee arthrotomy but no ACLT or ESWT. Group II underwent ACLT, but no ESWT. Group III underwent ACLT and received ESWT. The animalswerekilledat12weeks,andtheharvestedkneespec- imens were subjected to histo pathological examination and immune histo chemical analysis. Radiographs of the knees were obtained at 0 and 12 weeks. At 12 weeks, radiographs of group II showed more arthritic changes with formation of osteochondral fragments, whereas very subtle arthritis was noted in groups I and III. In histopathological examination, group II showed a significant increase of Mankin score and a decrease of sub chondral bone as compared to groups I and III. Group III showed a significant decrease of Mankin score and an increase of subchondral bone, with the data comparable to group I. In immunohistochemical analysis, group II showed significant increases of DKK-1 and MMP13 and decreases of Wnt-5a and β-catenin in articular cartilage and subchondral bone as compared to groups I and III. Group III showed sig-nificant decreases of DKK-1 and MMP13 and increases of Wnt-5a and β-catenin, with the data comparable to group I. In conclusion, the application of ESWT causes molecular changes that are consistent with the improvement in sub-chondral bone remodeling and chondroprotective effect in ACLT OA knees in rats.",

keywords = "ACLT, ESWT, Molecular change, OA knee, Rats",

author = "Wang, {C. J.} and Sun, {Y. C.} and Wu, {C. T.} and Weng, {L. H.} and Wang, {F. S.}",

note = "Publisher Copyright: {\textcopyright} Springer-Verlag Berlin Heidelberg 2013.",

year = "2016",

month = jan,

doi = "10.1007/s00193-013-0480-5",

language = "英语",

volume = "26",

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T1 - Molecular changes after shockwave therapy in osteoarthritic knee in rats

AU - Wang, C. J.

AU - Sun, Y. C.

AU - Wu, C. T.

AU - Weng, L. H.

AU - Wang, F. S.

N1 - Publisher Copyright: © Springer-Verlag Berlin Heidelberg 2013.

PY - 2016/1

Y1 - 2016/1

N2 - This study investigated the molecular changes of DKK-1, MMP13, Wnt-5a and β-catenin after extracorporeal shock wave therapy (ESWT) in anterior cruciateligament transected (ACLT) osteoarthritic (OA) knee in rats. 27 male Spraque-Dawley rats were divided into three groups. Group I was the control one and received sham knee arthrotomy but no ACLT or ESWT. Group II underwent ACLT, but no ESWT. Group III underwent ACLT and received ESWT. The animalswerekilledat12weeks,andtheharvestedkneespec- imens were subjected to histo pathological examination and immune histo chemical analysis. Radiographs of the knees were obtained at 0 and 12 weeks. At 12 weeks, radiographs of group II showed more arthritic changes with formation of osteochondral fragments, whereas very subtle arthritis was noted in groups I and III. In histopathological examination, group II showed a significant increase of Mankin score and a decrease of sub chondral bone as compared to groups I and III. Group III showed a significant decrease of Mankin score and an increase of subchondral bone, with the data comparable to group I. In immunohistochemical analysis, group II showed significant increases of DKK-1 and MMP13 and decreases of Wnt-5a and β-catenin in articular cartilage and subchondral bone as compared to groups I and III. Group III showed sig-nificant decreases of DKK-1 and MMP13 and increases of Wnt-5a and β-catenin, with the data comparable to group I. In conclusion, the application of ESWT causes molecular changes that are consistent with the improvement in sub-chondral bone remodeling and chondroprotective effect in ACLT OA knees in rats.

AB - This study investigated the molecular changes of DKK-1, MMP13, Wnt-5a and β-catenin after extracorporeal shock wave therapy (ESWT) in anterior cruciateligament transected (ACLT) osteoarthritic (OA) knee in rats. 27 male Spraque-Dawley rats were divided into three groups. Group I was the control one and received sham knee arthrotomy but no ACLT or ESWT. Group II underwent ACLT, but no ESWT. Group III underwent ACLT and received ESWT. The animalswerekilledat12weeks,andtheharvestedkneespec- imens were subjected to histo pathological examination and immune histo chemical analysis. Radiographs of the knees were obtained at 0 and 12 weeks. At 12 weeks, radiographs of group II showed more arthritic changes with formation of osteochondral fragments, whereas very subtle arthritis was noted in groups I and III. In histopathological examination, group II showed a significant increase of Mankin score and a decrease of sub chondral bone as compared to groups I and III. Group III showed a significant decrease of Mankin score and an increase of subchondral bone, with the data comparable to group I. In immunohistochemical analysis, group II showed significant increases of DKK-1 and MMP13 and decreases of Wnt-5a and β-catenin in articular cartilage and subchondral bone as compared to groups I and III. Group III showed sig-nificant decreases of DKK-1 and MMP13 and increases of Wnt-5a and β-catenin, with the data comparable to group I. In conclusion, the application of ESWT causes molecular changes that are consistent with the improvement in sub-chondral bone remodeling and chondroprotective effect in ACLT OA knees in rats.

KW - ACLT

KW - ESWT

KW - Molecular change

KW - OA knee

KW - Rats

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U2 - 10.1007/s00193-013-0480-5

DO - 10.1007/s00193-013-0480-5

M3 - 文章

AN - SCOPUS:85027942378

SN - 0938-1287

VL - 26

SP - 45

EP - 51

JO - Shock Waves

JF - Shock Waves

IS - 1

ER -

Molecular changes after shockwave therapy in osteoarthritic knee in rats

Abstract

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Keywords

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