Molecular epidemiological study of dengue virus type 1 in Taiwan

Kao Pin Hwang; Pei Yu Chu; Yi Ching Tung; Heng Lin Wang; Yi Yun Yueh; Ying Chang Wu; Chuan Chin; Kuei Hsiang Lin

doi:10.1002/jmv.10410

Molecular epidemiological study of dengue virus type 1 in Taiwan

Kao Pin Hwang, Pei Yu Chu, Yi Ching Tung, Heng Lin Wang, Yi Yun Yueh, Ying Chang Wu, Chuan Chin, Kuei Hsiang Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

11 Scopus citations

Abstract

Taiwan has experienced several major outbreaks of dengue (DEN) virus since 1981. The predominant virus type involved has been dengue virus type one (DEN-1), which first appeared in 1987. To understand the molecular epidemiology of this virus, 15 strains of DEN-1 isolated during 1987-1991 and 1994-1995, including 11 epidemic strains, two sporadic strains, and two imported strains have been studied. Fragments of 490 nucleotides (nt) from the E/NS1 junction were amplified by reverse transcription-polymerase chain reaction and the nt sequences were determined. Of the 490 nt of the E/NS1 junction, 240 nt (nt 2282-2521) were aligned and compared. Nucleotide substitutions were found at 54 positions among 15 isolates. Most nt changes were synonymous substitutions, and only three amino acid changes were found. A total of 61 strains isolated worldwide were analyzed by the Neighbor-joining method, and separated phylogenetically into three distinct genotypes, I-III. Genotype I comprised isolates from Japan and Hawaii collected in the 1940s. Genotype II included most strains isolated from Asia in 1977-1995. Genotype III consisted of isolates from three continents in 1964-1995: Asia, the Americas, and Africa. Genotype III was divided further into two subgenotypes, IIIA and IIIB. Most recent isolates from Taiwan, except for the sporadic strain isolated in 1995, were similar genetically and have been classified as Genotype II.

Original language	English
Pages (from-to)	404-409
Number of pages	6
Journal	Journal of Medical Virology
Volume	70
Issue number	3
DOIs	https://doi.org/10.1002/jmv.10410
State	Published - 01 07 2003
Externally published	Yes

Keywords

DEN-1
Genotype

Access to Document

10.1002/jmv.10410

Cite this

@article{01c3a3509a73468896a6d1c0e245d76f,

title = "Molecular epidemiological study of dengue virus type 1 in Taiwan",

abstract = "Taiwan has experienced several major outbreaks of dengue (DEN) virus since 1981. The predominant virus type involved has been dengue virus type one (DEN-1), which first appeared in 1987. To understand the molecular epidemiology of this virus, 15 strains of DEN-1 isolated during 1987-1991 and 1994-1995, including 11 epidemic strains, two sporadic strains, and two imported strains have been studied. Fragments of 490 nucleotides (nt) from the E/NS1 junction were amplified by reverse transcription-polymerase chain reaction and the nt sequences were determined. Of the 490 nt of the E/NS1 junction, 240 nt (nt 2282-2521) were aligned and compared. Nucleotide substitutions were found at 54 positions among 15 isolates. Most nt changes were synonymous substitutions, and only three amino acid changes were found. A total of 61 strains isolated worldwide were analyzed by the Neighbor-joining method, and separated phylogenetically into three distinct genotypes, I-III. Genotype I comprised isolates from Japan and Hawaii collected in the 1940s. Genotype II included most strains isolated from Asia in 1977-1995. Genotype III consisted of isolates from three continents in 1964-1995: Asia, the Americas, and Africa. Genotype III was divided further into two subgenotypes, IIIA and IIIB. Most recent isolates from Taiwan, except for the sporadic strain isolated in 1995, were similar genetically and have been classified as Genotype II.",

keywords = "DEN-1, Genotype",

author = "Hwang, {Kao Pin} and Chu, {Pei Yu} and Tung, {Yi Ching} and Wang, {Heng Lin} and Yueh, {Yi Yun} and Wu, {Ying Chang} and Chuan Chin and Lin, {Kuei Hsiang}",

year = "2003",

month = jul,

day = "1",

doi = "10.1002/jmv.10410",

language = "英语",

volume = "70",

pages = "404--409",

journal = "Journal of Medical Virology",

issn = "0146-6615",

publisher = "John Wiley and Sons Inc",

number = "3",

}

TY - JOUR

T1 - Molecular epidemiological study of dengue virus type 1 in Taiwan

AU - Hwang, Kao Pin

AU - Chu, Pei Yu

AU - Tung, Yi Ching

AU - Wang, Heng Lin

AU - Yueh, Yi Yun

AU - Wu, Ying Chang

AU - Chin, Chuan

AU - Lin, Kuei Hsiang

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Taiwan has experienced several major outbreaks of dengue (DEN) virus since 1981. The predominant virus type involved has been dengue virus type one (DEN-1), which first appeared in 1987. To understand the molecular epidemiology of this virus, 15 strains of DEN-1 isolated during 1987-1991 and 1994-1995, including 11 epidemic strains, two sporadic strains, and two imported strains have been studied. Fragments of 490 nucleotides (nt) from the E/NS1 junction were amplified by reverse transcription-polymerase chain reaction and the nt sequences were determined. Of the 490 nt of the E/NS1 junction, 240 nt (nt 2282-2521) were aligned and compared. Nucleotide substitutions were found at 54 positions among 15 isolates. Most nt changes were synonymous substitutions, and only three amino acid changes were found. A total of 61 strains isolated worldwide were analyzed by the Neighbor-joining method, and separated phylogenetically into three distinct genotypes, I-III. Genotype I comprised isolates from Japan and Hawaii collected in the 1940s. Genotype II included most strains isolated from Asia in 1977-1995. Genotype III consisted of isolates from three continents in 1964-1995: Asia, the Americas, and Africa. Genotype III was divided further into two subgenotypes, IIIA and IIIB. Most recent isolates from Taiwan, except for the sporadic strain isolated in 1995, were similar genetically and have been classified as Genotype II.

AB - Taiwan has experienced several major outbreaks of dengue (DEN) virus since 1981. The predominant virus type involved has been dengue virus type one (DEN-1), which first appeared in 1987. To understand the molecular epidemiology of this virus, 15 strains of DEN-1 isolated during 1987-1991 and 1994-1995, including 11 epidemic strains, two sporadic strains, and two imported strains have been studied. Fragments of 490 nucleotides (nt) from the E/NS1 junction were amplified by reverse transcription-polymerase chain reaction and the nt sequences were determined. Of the 490 nt of the E/NS1 junction, 240 nt (nt 2282-2521) were aligned and compared. Nucleotide substitutions were found at 54 positions among 15 isolates. Most nt changes were synonymous substitutions, and only three amino acid changes were found. A total of 61 strains isolated worldwide were analyzed by the Neighbor-joining method, and separated phylogenetically into three distinct genotypes, I-III. Genotype I comprised isolates from Japan and Hawaii collected in the 1940s. Genotype II included most strains isolated from Asia in 1977-1995. Genotype III consisted of isolates from three continents in 1964-1995: Asia, the Americas, and Africa. Genotype III was divided further into two subgenotypes, IIIA and IIIB. Most recent isolates from Taiwan, except for the sporadic strain isolated in 1995, were similar genetically and have been classified as Genotype II.

KW - DEN-1

KW - Genotype

UR - http://www.scopus.com/inward/record.url?scp=0038024171&partnerID=8YFLogxK

U2 - 10.1002/jmv.10410

DO - 10.1002/jmv.10410

M3 - 文章

C2 - 12767004

AN - SCOPUS:0038024171

SN - 0146-6615

VL - 70

SP - 404

EP - 409

JO - Journal of Medical Virology

JF - Journal of Medical Virology

IS - 3

ER -

Molecular epidemiological study of dengue virus type 1 in Taiwan

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this