Multiplex amplified product-length polymorphism analysis of 36 mitochondrial single-nucleotide polymorphisms for haplogrouping of East Asian populations

Kazuo Umetsu; Masashi Tanaka; Isao Yuasa; Noboru Adachi; Aya Miyoshi; Seiichi Kashimura; Kyung Sook Park; Yau Huei Wei; Gotaro Watanabe; Motoki Osawa

doi:10.1002/elps.200406129

Multiplex amplified product-length polymorphism analysis of 36 mitochondrial single-nucleotide polymorphisms for haplogrouping of East Asian populations

Kazuo Umetsu^*
, Masashi Tanaka
, Isao Yuasa
, Noboru Adachi
, Aya Miyoshi
, Seiichi Kashimura
, Kyung Sook Park
, Yau Huei Wei
, Gotaro Watanabe
, Motoki Osawa

^*Corresponding author for this work

Yamagata University
Gifu Intl. Inst. of Biotechnology
Tottori University
Tohoku University
Fukuoka University
Sungshin Women's University
National Yang Ming Chiao Tung University

Research output: Contribution to journal › Journal Article › peer-review

76 Scopus citations

Abstract

We present a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the haplogroup-diagnostic mitochondrial single-nucleotide polymorphisms (mtSNPs) in East Asian populations. By examining only 36 haplogroup-specific mtSNPs in the coding region by using four 9-multiplex polymerase chain reaction (PCR) and subsequent electrophoresis, we could safely assign 1815 individuals from 8 populations of Japanese, Korean, Chinese, and Germans to 45 relevant haplogroups. This multiplex APLP analysis of coding-region MtSNPs for haplogrouping is especially useful not only for molecular phylogenetic studies but also for large-scale association studies due to its rapid and economical nature. This is the first panel of mtSNPs in the coding region to be used for haplogrouping of East Asian populations.

Original language	English
Pages (from-to)	91-98
Number of pages	8
Journal	Electrophoresis
Volume	26
Issue number	1
DOIs	https://doi.org/10.1002/elps.200406129
State	Published - 01 2005
Externally published	Yes

Keywords

Amplified product-length polymorphism
Haplogroup
Mitochondrial DNA
Mitochondrial single-nucleotide polymorphism

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10.1002/elps.200406129

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title = "Multiplex amplified product-length polymorphism analysis of 36 mitochondrial single-nucleotide polymorphisms for haplogrouping of East Asian populations",

abstract = "We present a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the haplogroup-diagnostic mitochondrial single-nucleotide polymorphisms (mtSNPs) in East Asian populations. By examining only 36 haplogroup-specific mtSNPs in the coding region by using four 9-multiplex polymerase chain reaction (PCR) and subsequent electrophoresis, we could safely assign 1815 individuals from 8 populations of Japanese, Korean, Chinese, and Germans to 45 relevant haplogroups. This multiplex APLP analysis of coding-region MtSNPs for haplogrouping is especially useful not only for molecular phylogenetic studies but also for large-scale association studies due to its rapid and economical nature. This is the first panel of mtSNPs in the coding region to be used for haplogrouping of East Asian populations.",

keywords = "Amplified product-length polymorphism, Haplogroup, Mitochondrial DNA, Mitochondrial single-nucleotide polymorphism",

author = "Kazuo Umetsu and Masashi Tanaka and Isao Yuasa and Noboru Adachi and Aya Miyoshi and Seiichi Kashimura and Park, \{Kyung Sook\} and Wei, \{Yau Huei\} and Gotaro Watanabe and Motoki Osawa",

year = "2005",

month = jan,

doi = "10.1002/elps.200406129",

language = "英语",

volume = "26",

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publisher = "John Wiley and Sons Inc",

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T1 - Multiplex amplified product-length polymorphism analysis of 36 mitochondrial single-nucleotide polymorphisms for haplogrouping of East Asian populations

AU - Umetsu, Kazuo

AU - Tanaka, Masashi

AU - Yuasa, Isao

AU - Adachi, Noboru

AU - Miyoshi, Aya

AU - Kashimura, Seiichi

AU - Park, Kyung Sook

AU - Wei, Yau Huei

AU - Watanabe, Gotaro

AU - Osawa, Motoki

PY - 2005/1

Y1 - 2005/1

N2 - We present a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the haplogroup-diagnostic mitochondrial single-nucleotide polymorphisms (mtSNPs) in East Asian populations. By examining only 36 haplogroup-specific mtSNPs in the coding region by using four 9-multiplex polymerase chain reaction (PCR) and subsequent electrophoresis, we could safely assign 1815 individuals from 8 populations of Japanese, Korean, Chinese, and Germans to 45 relevant haplogroups. This multiplex APLP analysis of coding-region MtSNPs for haplogrouping is especially useful not only for molecular phylogenetic studies but also for large-scale association studies due to its rapid and economical nature. This is the first panel of mtSNPs in the coding region to be used for haplogrouping of East Asian populations.

AB - We present a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the haplogroup-diagnostic mitochondrial single-nucleotide polymorphisms (mtSNPs) in East Asian populations. By examining only 36 haplogroup-specific mtSNPs in the coding region by using four 9-multiplex polymerase chain reaction (PCR) and subsequent electrophoresis, we could safely assign 1815 individuals from 8 populations of Japanese, Korean, Chinese, and Germans to 45 relevant haplogroups. This multiplex APLP analysis of coding-region MtSNPs for haplogrouping is especially useful not only for molecular phylogenetic studies but also for large-scale association studies due to its rapid and economical nature. This is the first panel of mtSNPs in the coding region to be used for haplogrouping of East Asian populations.

KW - Amplified product-length polymorphism

KW - Haplogroup

KW - Mitochondrial DNA

KW - Mitochondrial single-nucleotide polymorphism

UR - https://www.scopus.com/pages/publications/20844461653

U2 - 10.1002/elps.200406129

DO - 10.1002/elps.200406129

M3 - 文章

C2 - 15624129

AN - SCOPUS:20844461653

SN - 0173-0835

VL - 26

SP - 91

EP - 98

JO - Electrophoresis

JF - Electrophoresis

IS - 1

ER -

Multiplex amplified product-length polymorphism analysis of 36 mitochondrial single-nucleotide polymorphisms for haplogrouping of East Asian populations

Abstract

Keywords

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