Nanofibrous insulin/vildagliptin core-shell PLGA scaffold promotes diabetic wound healing

Chen Hung Lee*, Dong Yi Chen, Ming Jer Hsieh, Kuo Chun Hung, Shu Chun Huang, Chia Jung Cho*, Shih Jung Liu*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

8 Scopus citations

Abstract

Introduction: Slow wound repair in diabetes is a serious adverse event that often results in loss of a limb or disability. An advanced and encouraging vehicle is wanted to enhance clinically applicable diabetic wound care. Nanofibrous insulin/vildagliptin core-shell biodegradable poly (lactic-co-glycolic acid) (PLGA) scaffolds to prolong the effective drug delivery of vildagliptin and insulin for the repair of diabetic wounds were prepared. Methods: To fabricate core-shell nanofibrous membranes, vildagliptin mixture with PLGA, and insulin solution were pumped via separate pumps into two differently sized capillary tubes that were coaxially electrospun. Results and Discussion: Nanofibrous core-shell scaffolds slowly released effective vildagliptin and insulin over 2 weeks in vitro migration assay and in vivo wound-healing models. Water contact angle (68.3 ± 8.5° vs. 121.4 ± 2.0°, p = 0.006) and peaked water absorbent capacity (376% ± 9% vs. 283% ± 24%, p = 0.003) of the insulin/vildagliptin core-shell nanofibrous membranes remarkably exceeded those of a control group. The insulin/vildagliptin-loaded core-shell nanofibers improved endothelial progenitor cells migration in vitro (762 ± 77 cells/mm 2 vs. 424.4 ± 23 cells/mm 2, p < 0.001), reduced the α-smooth muscle actin content in vivo (0.72 ± 0.23 vs. 2.07 ± 0.37, p < 0.001), and increased diabetic would recovery (1.9 ± 0.3 mm 2 vs. 8.0 ± 1.4 mm 2, p = 0.002). Core-shell insulin/vildagliptin-loaded nanofibers extend the drug delivery of insulin and vildagliptin and accelerate the repair of wounds associated with diabetes.

Original languageEnglish
Article number1075720
Pages (from-to)1075720
JournalFrontiers in Bioengineering and Biotechnology
Volume11
DOIs
StatePublished - 2023

Bibliographical note

Copyright © 2023 Lee, Chen, Hsieh, Hung, Huang, Cho and Liu.

Keywords

  • core-shell nanofiber
  • diabetes
  • insulin
  • vildagliptin
  • wound

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