Nanoparticle-enhanced postbiotics: Revolutionizing cancer therapy through effective delivery

Hau Lun Huang, Chih Ho Lai, Wan Hua Tsai, Kuo Wei Chen, Shin Lei Peng, Jui Hsiang Lin, Yu Hsin Lin*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

2 Scopus citations


Aim: Gastric cancer contributes to cancer-related fatalities. Conventional chemotherapy faces challenges due to severe adverse effects, prompting recent research to focus on postbiotics, which are safer biomolecules derived from nonviable probiotics. Despite promising in vitro results, efficient in vivo delivery systems remain a challenge. This study aimed to design a potential nanoparticle (NP) formulation encapsulating the Lacticaseibacillus paracasei GMNL-133 (SGMNL-133) isolate to enhance its therapeutic efficacy in treating gastric cancer. Main methods: We successfully isolated GMNL-133 (SGMNL-133) by optimizing the lysate extraction and column elution processes for L. paracasei GMNL-133, resulting in substantial enhancement of its capacity to inhibit the proliferation of gastric cancer cells. Additionally, we developed a potential NP utilizing arginine-chitosan and fucoidan encapsulating SGMNL-133. Key findings: This innovative approach protected the SGMNL-133 from degradation by gastric acid, facilitated its penetration through the mucus layer, and enabled interaction with gastric cancer cells. Furthermore, in vivo experiments demonstrated that the encapsulation of SGMNL-133 in NPs significantly enhanced its efficacy in the treatment of orthotopic gastric tumors while simultaneously reducing tissue inflammation levels. Significance: Recent research highlights postbiotics as a safe alternative, but in vivo delivery remains a challenge. Our study optimized the extraction of the lysate and column elution of GMNL-133, yielding SGMNL-133. We also developed NPs to protect SGMNL-133 from gastric acid, enhance mucus penetration, and improve the interaction with gastric cancer cells. This combination significantly enhanced drug delivery and anti-gastric tumor activity.

Original languageEnglish
Article number122379
Pages (from-to)122379
JournalLife Sciences
StatePublished - 15 01 2024
Externally publishedYes

Bibliographical note

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.


  • Arginine-chitosan
  • Fucoidan
  • Nanoparticle
  • Postbiotics
  • SGMNL133
  • Stomach Neoplasms/drug therapy
  • Nanoparticles
  • Humans
  • Cell Line, Tumor
  • Drug Delivery Systems/methods


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