Nanoparticles-based electrochemical biosensor for single bacterium detection by redox signal amplification

Chih Shuan Lu, Po Chao Wen, Hwan You Chang, Fan Gang Tseng*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

In this work, we report a portable mierofluidie chip based on Si-Porous-Pt MSNs(Mesoporous Silica Nanoparticles) -enhanced electrochemical detection for single-bacteria diognostic. The possibility of single-bacteria detection is realized here by using electroactivc molecules modified on Si-Porous-Pt MSNs(Mcsoporous Silica Nanoparticles) to detect and differentiate Staphylococcus aureus and Pseudomonas aeruginosa. The detection limit of these two electroactivc molecules is near femtomolar (1 pM) when immobilized on Si-Porous-Pt MSNs(Mesoporous Silica Nanoparticles), and the minima amounts of gold NPs required in the detection is around thousands (103 particle/μl). A concentration of couples whole bacteria (<10 cells/μ1) is detected by CV measurements. The liner detection limit conccntion is form 1- 1000 cells/μl. The results showed that the portable biochip system has great potential as a device for single -particle or possibly even single-organism detection.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages239-241
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 10 201331 10 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/1331/10/13

Keywords

  • Redox signal amplification
  • Si-Porous-Pt MSNs(mcsoporous silica nanoparticlcs)
  • Singlc bacteria detection

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