Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury

Ting Hsu Chen; Chien Chong Hong; Tong Miin Liu; Chian Lang Hong; Chung Hang Wang; Chih Chung Lin; Yun Ching Huang

Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury

Ting Hsu Chen^*, Chien Chong Hong, Tong Miin Liu, Chian Lang Hong, Chung Hang Wang, Chih Chung Lin, Yun Ching Huang

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In this paper, we present novel electrical microfluidic biosensors with artificially-made recognition elements for detection of Neutrophil gelatinase-associated lipocalin (NGAL) protein, which is a biomarker of acute renal failure (AKI). By using molecularly-imprinted polymer electricpolimerized on electrode, we can identify different concentration of NGAL protein. The results shows that in the specific region of current density and voltage, the polymerized electrodes can distinguish different concentrations of protein well. It can also recognize concentrations in ppb level(50~600 ng/mL). Also, the performance of sweeping frequency reveals low frequency can show more capacitance difference in all concentration, may promote the detect efficiency.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Publisher	Chemical and Biological Microsystems Society
Pages	832-834
Number of pages	3
ISBN (Electronic)	9781510897571
State	Published - 2018
Externally published	Yes
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan Duration: 11 11 2018 → 15 11 2018

Publication series

Name	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume	2

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/Territory	Taiwan
City	Kaohsiung
Period	11/11/18 → 15/11/18

Bibliographical note

Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.

Keywords

Acute kidney injury
Electrical biosensor
Microfluidic chip
Molecularly-imprinted polymer
Neutrophil gelatinase-associated lipocalin protein

Cite this

Chen, T. H., Hong, C. C., Liu, T. M., Hong, C. L., Wang, C. H., Lin, C. C., & Huang, Y. C. (2018). Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 832-834). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 2). Chemical and Biological Microsystems Society.

Chen, Ting Hsu ; Hong, Chien Chong ; Liu, Tong Miin et al. / Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. pp. 832-834 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

@inproceedings{3e867428aaf241289eddd44f3dc88b27,

title = "Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury",

abstract = "In this paper, we present novel electrical microfluidic biosensors with artificially-made recognition elements for detection of Neutrophil gelatinase-associated lipocalin (NGAL) protein, which is a biomarker of acute renal failure (AKI). By using molecularly-imprinted polymer electricpolimerized on electrode, we can identify different concentration of NGAL protein. The results shows that in the specific region of current density and voltage, the polymerized electrodes can distinguish different concentrations of protein well. It can also recognize concentrations in ppb level(50~600 ng/mL). Also, the performance of sweeping frequency reveals low frequency can show more capacitance difference in all concentration, may promote the detect efficiency.",

keywords = "Acute kidney injury, Electrical biosensor, Microfluidic chip, Molecularly-imprinted polymer, Neutrophil gelatinase-associated lipocalin protein",

author = "Chen, {Ting Hsu} and Hong, {Chien Chong} and Liu, {Tong Miin} and Hong, {Chian Lang} and Wang, {Chung Hang} and Lin, {Chih Chung} and Huang, {Yun Ching}",

note = "Publisher Copyright: Copyright {\textcopyright} (2018) by Chemical and Biological Microsystems Society. All rights reserved.; 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

year = "2018",

language = "英语",

series = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

publisher = "Chemical and Biological Microsystems Society",

pages = "832--834",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

}

Chen, TH, Hong, CC, Liu, TM, Hong, CL, Wang, CH, Lin, CC & Huang, YC 2018, Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, vol. 2, Chemical and Biological Microsystems Society, pp. 832-834, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, Taiwan, 11/11/18.

Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury. / Chen, Ting Hsu; Hong, Chien Chong; Liu, Tong Miin et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society, 2018. p. 832-834 (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury

AU - Chen, Ting Hsu

AU - Hong, Chien Chong

AU - Liu, Tong Miin

AU - Hong, Chian Lang

AU - Wang, Chung Hang

AU - Lin, Chih Chung

AU - Huang, Yun Ching

PY - 2018

Y1 - 2018

N2 - In this paper, we present novel electrical microfluidic biosensors with artificially-made recognition elements for detection of Neutrophil gelatinase-associated lipocalin (NGAL) protein, which is a biomarker of acute renal failure (AKI). By using molecularly-imprinted polymer electricpolimerized on electrode, we can identify different concentration of NGAL protein. The results shows that in the specific region of current density and voltage, the polymerized electrodes can distinguish different concentrations of protein well. It can also recognize concentrations in ppb level(50~600 ng/mL). Also, the performance of sweeping frequency reveals low frequency can show more capacitance difference in all concentration, may promote the detect efficiency.

AB - In this paper, we present novel electrical microfluidic biosensors with artificially-made recognition elements for detection of Neutrophil gelatinase-associated lipocalin (NGAL) protein, which is a biomarker of acute renal failure (AKI). By using molecularly-imprinted polymer electricpolimerized on electrode, we can identify different concentration of NGAL protein. The results shows that in the specific region of current density and voltage, the polymerized electrodes can distinguish different concentrations of protein well. It can also recognize concentrations in ppb level(50~600 ng/mL). Also, the performance of sweeping frequency reveals low frequency can show more capacitance difference in all concentration, may promote the detect efficiency.

KW - Acute kidney injury

KW - Electrical biosensor

KW - Microfluidic chip

KW - Molecularly-imprinted polymer

KW - Neutrophil gelatinase-associated lipocalin protein

UR - http://www.scopus.com/inward/record.url?scp=85079866065&partnerID=8YFLogxK

M3 - 会议稿件

AN - SCOPUS:85079866065

T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

SP - 832

EP - 834

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

Y2 - 11 November 2018 through 15 November 2018

ER -

Chen TH, Hong CC, Liu TM, Hong CL, Wang CH, Lin CC et al. Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Chemical and Biological Microsystems Society. 2018. p. 832-834. (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

Neutrophil gelatinase-associated lipocalin protein biosensors based on artificially-made recognition elements for rapid diagnostics of acute kidney injury

Abstract

Publication series

Conference

Bibliographical note

Keywords

Other files and links

Fingerprint

Cite this