Nitrogen-doped carbon nanodots prepared from polyethylenimine for fluorometric determination of salivary uric acid

Wei Cheng Wu; Hsin Yi Tiffany Chen; Shih Chi Lin; Hsin Ying Chen; Fu Rong Chen; Huan Tsung Chang; Fan Gang Tseng

doi:10.1007/s00604-019-3277-0

Nitrogen-doped carbon nanodots prepared from polyethylenimine for fluorometric determination of salivary uric acid

Wei Cheng Wu
, Hsin Yi Tiffany Chen
, Shih Chi Lin
, Hsin Ying Chen
, Fu Rong Chen
, Huan Tsung Chang
, Fan Gang Tseng^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

37 Scopus citations

Abstract

Stable and low-cost carbon dots (C-dots) were prepared from polyethylenimine (PEI) by a hydrothermal method. It is found that the fluorescence of the C-dots (best measured at excitation/emission wavelengths of 365/473 nm) is quenched by selective oxidation of surface PEI by periodate but recovers in the presence of uric acid (UA). It is assumed that this is due to the selective reduction of the nitrone groups to hydroxylamine groups by UA. The findings were used to design a fluorometric method for determination of UA that has a 2.3 nM detection limit. This is lower than that of reported fluorometric and enzymatic assays. The performance of the method has been validated by determination of UA in samples of human saliva. It is found that the results agree well with those obtained by a commercial UA assay.

Original language	English
Article number	166
Journal	Microchimica Acta
Volume	186
Issue number	3
DOIs	https://doi.org/10.1007/s00604-019-3277-0
State	Published - 01 03 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019, Springer-Verlag GmbH Austria, part of Springer Nature.

Keywords

Antioxidant
C-dots
Hydrothermal synthesis
Nitrone
Periodate
Uricase

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10.1007/s00604-019-3277-0

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title = "Nitrogen-doped carbon nanodots prepared from polyethylenimine for fluorometric determination of salivary uric acid",

abstract = "Stable and low-cost carbon dots (C-dots) were prepared from polyethylenimine (PEI) by a hydrothermal method. It is found that the fluorescence of the C-dots (best measured at excitation/emission wavelengths of 365/473 nm) is quenched by selective oxidation of surface PEI by periodate but recovers in the presence of uric acid (UA). It is assumed that this is due to the selective reduction of the nitrone groups to hydroxylamine groups by UA. The findings were used to design a fluorometric method for determination of UA that has a 2.3 nM detection limit. This is lower than that of reported fluorometric and enzymatic assays. The performance of the method has been validated by determination of UA in samples of human saliva. It is found that the results agree well with those obtained by a commercial UA assay.",

keywords = "Antioxidant, C-dots, Hydrothermal synthesis, Nitrone, Periodate, Uricase",

author = "Wu, \{Wei Cheng\} and Chen, \{Hsin Yi Tiffany\} and Lin, \{Shih Chi\} and Chen, \{Hsin Ying\} and Chen, \{Fu Rong\} and Chang, \{Huan Tsung\} and Tseng, \{Fan Gang\}",

note = "Publisher Copyright: {\textcopyright} 2019, Springer-Verlag GmbH Austria, part of Springer Nature.",

year = "2019",

month = mar,

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doi = "10.1007/s00604-019-3277-0",

language = "英语",

volume = "186",

journal = "Microchimica Acta",

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T1 - Nitrogen-doped carbon nanodots prepared from polyethylenimine for fluorometric determination of salivary uric acid

AU - Wu, Wei Cheng

AU - Chen, Hsin Yi Tiffany

AU - Lin, Shih Chi

AU - Chen, Hsin Ying

AU - Chen, Fu Rong

AU - Chang, Huan Tsung

AU - Tseng, Fan Gang

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Stable and low-cost carbon dots (C-dots) were prepared from polyethylenimine (PEI) by a hydrothermal method. It is found that the fluorescence of the C-dots (best measured at excitation/emission wavelengths of 365/473 nm) is quenched by selective oxidation of surface PEI by periodate but recovers in the presence of uric acid (UA). It is assumed that this is due to the selective reduction of the nitrone groups to hydroxylamine groups by UA. The findings were used to design a fluorometric method for determination of UA that has a 2.3 nM detection limit. This is lower than that of reported fluorometric and enzymatic assays. The performance of the method has been validated by determination of UA in samples of human saliva. It is found that the results agree well with those obtained by a commercial UA assay.

AB - Stable and low-cost carbon dots (C-dots) were prepared from polyethylenimine (PEI) by a hydrothermal method. It is found that the fluorescence of the C-dots (best measured at excitation/emission wavelengths of 365/473 nm) is quenched by selective oxidation of surface PEI by periodate but recovers in the presence of uric acid (UA). It is assumed that this is due to the selective reduction of the nitrone groups to hydroxylamine groups by UA. The findings were used to design a fluorometric method for determination of UA that has a 2.3 nM detection limit. This is lower than that of reported fluorometric and enzymatic assays. The performance of the method has been validated by determination of UA in samples of human saliva. It is found that the results agree well with those obtained by a commercial UA assay.

KW - Antioxidant

KW - C-dots

KW - Hydrothermal synthesis

KW - Nitrone

KW - Periodate

KW - Uricase

UR - https://www.scopus.com/pages/publications/85061280561

U2 - 10.1007/s00604-019-3277-0

DO - 10.1007/s00604-019-3277-0

M3 - 文章

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AN - SCOPUS:85061280561

SN - 0026-3672

VL - 186

JO - Microchimica Acta

JF - Microchimica Acta

IS - 3

M1 - 166

ER -

Nitrogen-doped carbon nanodots prepared from polyethylenimine for fluorometric determination of salivary uric acid

Abstract

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Keywords

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