Nocistatin and nociceptin exert opposite effects on the excitability of central amygdala nucleus-periaqueductal gray projection neurons

Ying Ling Chen; Allen H. Li; Tu Hsueh Yeh; An Hsun Chou; Hung Li Wang

doi:10.1016/j.mcn.2008.09.003

Nocistatin and nociceptin exert opposite effects on the excitability of central amygdala nucleus-periaqueductal gray projection neurons

Ying Ling Chen, Allen H. Li, Tu Hsueh Yeh, An Hsun Chou, Hung Li Wang^*

^*Corresponding author for this work

Department of Physiology

Research output: Contribution to journal › Journal Article › peer-review

26 Scopus citations

Abstract

Central amygdala nucleus (CeA)-periaqueductal gray (PAG) pathway is the component of descending antinociceptive circuitry. Nociceptin/orphanin FQ (N/OFQ) and nocistatin (NST) produce supraspinal pronociceptive and antinociceptive effects, respectively. We hypothesized that opposite effects of N/OFQ and NST on supraspinal pain modulation result from their opposing effects on the excitability of CeA-PAG projection neurons. This hypothesis was tested by investigating electrophysiological effects of N/OFQ and NST on medial CeA neurons that project to PAG (CeA_M-PAG). N/OFQ hyperpolarized CeA_M-PAG projection neurons by enhancing inwardly rectifying potassium conductance. In contrast, NST depolarized CeA_M-PAG neurons by causing the opening of TRPC cation channels via G_αq/11-PLC-PKC pathway. CeA_M-PAG neurons hyperpolarized by N/OFQ express CRF or neurotensin mRNA. NST-responsive CeA_M-PAG neurons contain CRF or substance P mRNA. Our study provides the evidence that the molecular and cellular basis for opposite effects of N/OFQ and NST on supraspinal pain regulation is their opposing effects on the excitability of peptidergic CeA_M-PAG neurons.

Original language	English
Pages (from-to)	76-88
Number of pages	13
Journal	Molecular and Cellular Neuroscience
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/j.mcn.2008.09.003
State	Published - 05 01 2009

Keywords

CeA-PAG projection neurons
Central amygdala nucleus
Nociceptin/orphanin FQ
Nocistatin
Periaqueductal gray

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10.1016/j.mcn.2008.09.003

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title = "Nocistatin and nociceptin exert opposite effects on the excitability of central amygdala nucleus-periaqueductal gray projection neurons",

abstract = "Central amygdala nucleus (CeA)-periaqueductal gray (PAG) pathway is the component of descending antinociceptive circuitry. Nociceptin/orphanin FQ (N/OFQ) and nocistatin (NST) produce supraspinal pronociceptive and antinociceptive effects, respectively. We hypothesized that opposite effects of N/OFQ and NST on supraspinal pain modulation result from their opposing effects on the excitability of CeA-PAG projection neurons. This hypothesis was tested by investigating electrophysiological effects of N/OFQ and NST on medial CeA neurons that project to PAG (CeAM-PAG). N/OFQ hyperpolarized CeAM-PAG projection neurons by enhancing inwardly rectifying potassium conductance. In contrast, NST depolarized CeAM-PAG neurons by causing the opening of TRPC cation channels via Gαq/11-PLC-PKC pathway. CeAM-PAG neurons hyperpolarized by N/OFQ express CRF or neurotensin mRNA. NST-responsive CeAM-PAG neurons contain CRF or substance P mRNA. Our study provides the evidence that the molecular and cellular basis for opposite effects of N/OFQ and NST on supraspinal pain regulation is their opposing effects on the excitability of peptidergic CeAM-PAG neurons.",

keywords = "CeA-PAG projection neurons, Central amygdala nucleus, Nociceptin/orphanin FQ, Nocistatin, Periaqueductal gray",

author = "Chen, {Ying Ling} and Li, {Allen H.} and Yeh, {Tu Hsueh} and Chou, {An Hsun} and Wang, {Hung Li}",

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T1 - Nocistatin and nociceptin exert opposite effects on the excitability of central amygdala nucleus-periaqueductal gray projection neurons

AU - Chen, Ying Ling

AU - Li, Allen H.

AU - Yeh, Tu Hsueh

AU - Chou, An Hsun

AU - Wang, Hung Li

PY - 2009/1/5

Y1 - 2009/1/5

N2 - Central amygdala nucleus (CeA)-periaqueductal gray (PAG) pathway is the component of descending antinociceptive circuitry. Nociceptin/orphanin FQ (N/OFQ) and nocistatin (NST) produce supraspinal pronociceptive and antinociceptive effects, respectively. We hypothesized that opposite effects of N/OFQ and NST on supraspinal pain modulation result from their opposing effects on the excitability of CeA-PAG projection neurons. This hypothesis was tested by investigating electrophysiological effects of N/OFQ and NST on medial CeA neurons that project to PAG (CeAM-PAG). N/OFQ hyperpolarized CeAM-PAG projection neurons by enhancing inwardly rectifying potassium conductance. In contrast, NST depolarized CeAM-PAG neurons by causing the opening of TRPC cation channels via Gαq/11-PLC-PKC pathway. CeAM-PAG neurons hyperpolarized by N/OFQ express CRF or neurotensin mRNA. NST-responsive CeAM-PAG neurons contain CRF or substance P mRNA. Our study provides the evidence that the molecular and cellular basis for opposite effects of N/OFQ and NST on supraspinal pain regulation is their opposing effects on the excitability of peptidergic CeAM-PAG neurons.

AB - Central amygdala nucleus (CeA)-periaqueductal gray (PAG) pathway is the component of descending antinociceptive circuitry. Nociceptin/orphanin FQ (N/OFQ) and nocistatin (NST) produce supraspinal pronociceptive and antinociceptive effects, respectively. We hypothesized that opposite effects of N/OFQ and NST on supraspinal pain modulation result from their opposing effects on the excitability of CeA-PAG projection neurons. This hypothesis was tested by investigating electrophysiological effects of N/OFQ and NST on medial CeA neurons that project to PAG (CeAM-PAG). N/OFQ hyperpolarized CeAM-PAG projection neurons by enhancing inwardly rectifying potassium conductance. In contrast, NST depolarized CeAM-PAG neurons by causing the opening of TRPC cation channels via Gαq/11-PLC-PKC pathway. CeAM-PAG neurons hyperpolarized by N/OFQ express CRF or neurotensin mRNA. NST-responsive CeAM-PAG neurons contain CRF or substance P mRNA. Our study provides the evidence that the molecular and cellular basis for opposite effects of N/OFQ and NST on supraspinal pain regulation is their opposing effects on the excitability of peptidergic CeAM-PAG neurons.

KW - CeA-PAG projection neurons

KW - Central amygdala nucleus

KW - Nociceptin/orphanin FQ

KW - Nocistatin

KW - Periaqueductal gray

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ER -

Nocistatin and nociceptin exert opposite effects on the excitability of central amygdala nucleus-periaqueductal gray projection neurons

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