Non-radioactive Southern hybridization for early diagnosis of α-thalassemia with Southeast Asian-type deletion in Taiwan

Da Chang Chu, Chien Hong Lee, Ming De Lo, Shu Wei Cheng, Ding Ping Chen, Tsu Lan Wu, Kuo Chien Tsao, Daniel T.Y. Chiu, Chien Feng Sun*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

7 Scopus citations

Abstract

α-Thalassemia has been estimated to account for over 60% of hydrops fetalis cases in Taiwan. The most common genotypic lesion found in α-thalassemia-1 cases in Taiwan is deletion of a large segment of the α-globin gene cluster, termed the Southeast Asian-type deletion (-(SEA)/; further referred to as SEA-type deletion). Seven chorionic villus samples (CVS) from pregnancies of couples both heterozygous for SEA-type deletion were studied. Non-radioactive Southern-blot hybridization using the digalkaline phosphatase detection system was developed to fulfill this purpose. The results were compared with corresponding polymerase chain reaction (PCR) data to elucidate the effectiveness of these two protocols in the diagnosis of the SEA-type deletion. The data showed that of the seven CVS, three demonstrated a distinctive band pattern, indicating their homozygous status of SEA-type deletion, whereas two showed heterozygous patterns, and the other two were free of the deletion. Homozygosity of the deletion was confirmed by Southern-blot hybridization performed on DNA samples extracted from the abortus tissue. However, two of the three cases with SEA-type deletion showed heterozygous PCR results. Maternal cell contamination could be responsible for the artifacts in the PCR results, but the influence due to the contamination is minimal in non-radioactive Southern-blot hybridization. We concluded that PCR is suitable for screening of carrier adults with SEA-type deletion, and non-radioactive Southern hybridization is ideal for early prenatal diagnosis of the SEA-type deletion. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)332-335
Number of pages4
JournalAmerican Journal of Medical Genetics
Volume95
Issue number4
DOIs
StatePublished - 11 12 2000

Keywords

  • Chorionic villus sampling
  • Non-radioactive Southern blotting
  • PCR
  • SEA-type deletion
  • α-thalassemia-1

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