Novel affinity-based processes for protein purification

Jyh Ping Chen

doi:10.1016/0922-338X(90)90188-3

Novel affinity-based processes for protein purification

Jyh Ping Chen^*

^*Corresponding author for this work

University of Wisconsin-Madison

Research output: Contribution to journal › Review article › peer-review

22 Scopus citations

Abstract

To circumvent the difficulties encountered in conventional affinity chromatography, novel affinity-based protein purification processes using reversible protein-ligand interactions have been developed. Depending on the physical means of isolating the ligand-ligate complex, the processes can be categorized as affinity precipitation, affinity cross-flow filtration, or affinity partition. This report reviews the current status of such purification techniques.

Original language	English
Pages (from-to)	199-209
Number of pages	11
Journal	Journal of Fermentation and Bioengineering
Volume	70
Issue number	3
DOIs	https://doi.org/10.1016/0922-338X(90)90188-3
State	Published - 1990
Externally published	Yes

Access to Document

10.1016/0922-338X(90)90188-3

Cite this

@article{66433c80e7a2465a8d2f245a9127e821,

title = "Novel affinity-based processes for protein purification",

abstract = "To circumvent the difficulties encountered in conventional affinity chromatography, novel affinity-based protein purification processes using reversible protein-ligand interactions have been developed. Depending on the physical means of isolating the ligand-ligate complex, the processes can be categorized as affinity precipitation, affinity cross-flow filtration, or affinity partition. This report reviews the current status of such purification techniques.",

author = "Chen, \{Jyh Ping\}",

year = "1990",

doi = "10.1016/0922-338X(90)90188-3",

language = "英语",

volume = "70",

pages = "199--209",

journal = "Journal of Fermentation and Bioengineering",

issn = "0922-338X",

publisher = "The Society of Bioscience and Bioengineering Japan/Seibutsu Kogakkai",

number = "3",

}

TY - JOUR

T1 - Novel affinity-based processes for protein purification

AU - Chen, Jyh Ping

PY - 1990

Y1 - 1990

N2 - To circumvent the difficulties encountered in conventional affinity chromatography, novel affinity-based protein purification processes using reversible protein-ligand interactions have been developed. Depending on the physical means of isolating the ligand-ligate complex, the processes can be categorized as affinity precipitation, affinity cross-flow filtration, or affinity partition. This report reviews the current status of such purification techniques.

AB - To circumvent the difficulties encountered in conventional affinity chromatography, novel affinity-based protein purification processes using reversible protein-ligand interactions have been developed. Depending on the physical means of isolating the ligand-ligate complex, the processes can be categorized as affinity precipitation, affinity cross-flow filtration, or affinity partition. This report reviews the current status of such purification techniques.

UR - https://www.scopus.com/pages/publications/0025052246

U2 - 10.1016/0922-338X(90)90188-3

DO - 10.1016/0922-338X(90)90188-3

M3 - 文献综述

AN - SCOPUS:0025052246

SN - 0922-338X

VL - 70

SP - 199

EP - 209

JO - Journal of Fermentation and Bioengineering

JF - Journal of Fermentation and Bioengineering

IS - 3

ER -

Novel affinity-based processes for protein purification

Abstract

Access to Document

Other files and links

Fingerprint

Cite this