Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

Sue Jane Lin; Shao Wen Wu; Ya Ching Chou; Jiun Han Lin; Ya Chi Huang; Mei Ru Chen; Nianhan Ma; Ching Hwa Tsai

doi:10.1016/j.virol.2015.02.015

Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

Sue Jane Lin
, Shao Wen Wu
, Ya Ching Chou
, Jiun Han Lin
, Ya Chi Huang
, Mei Ru Chen
, Nianhan Ma^*
, Ching Hwa Tsai

^*Corresponding author for this work

Department of Medical Biotechnology and Laboratory Science

National Taiwan University
National Central University

Research output: Contribution to journal › Journal Article › peer-review

10 Scopus citations

Abstract

Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.

Original language	English
Pages (from-to)	24-33
Number of pages	10
Journal	Virology
Volume	481
DOIs	https://doi.org/10.1016/j.virol.2015.02.015
State	Published - 01 07 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Inc.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
EBV
LCL
TIMP-1
Zta

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10.1016/j.virol.2015.02.015

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@article{87ff74e90c8a4c748a33c7ceb2fba4e8,

title = "Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival",

abstract = "Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.",

keywords = "Apoptosis, EBV, LCL, TIMP-1, Zta",

author = "Lin, \{Sue Jane\} and Wu, \{Shao Wen\} and Chou, \{Ya Ching\} and Lin, \{Jiun Han\} and Huang, \{Ya Chi\} and Chen, \{Mei Ru\} and Nianhan Ma and Tsai, \{Ching Hwa\}",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

year = "2015",

month = jul,

day = "1",

doi = "10.1016/j.virol.2015.02.015",

language = "英语",

volume = "481",

pages = "24--33",

journal = "Virology",

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T1 - Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

AU - Lin, Sue Jane

AU - Wu, Shao Wen

AU - Chou, Ya Ching

AU - Lin, Jiun Han

AU - Huang, Ya Chi

AU - Chen, Mei Ru

AU - Ma, Nianhan

AU - Tsai, Ching Hwa

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.

AB - Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.

KW - Apoptosis

KW - EBV

KW - LCL

KW - TIMP-1

KW - Zta

UR - https://www.scopus.com/pages/publications/84924296960

U2 - 10.1016/j.virol.2015.02.015

DO - 10.1016/j.virol.2015.02.015

M3 - 文章

C2 - 25765004

AN - SCOPUS:84924296960

SN - 0042-6822

VL - 481

SP - 24

EP - 33

JO - Virology

JF - Virology

ER -

Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

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