Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53

Hui Jia Chan; Jing Jei Weng; Benjamin Yat Ming Yung

doi:10.1016/j.bbrc.2005.04.176

Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53

Hui Jia Chan
, Jing Jei Weng
, Benjamin Yat Ming Yung^*

^*Corresponding author for this work

Graduate Institute of Biomedical Sciences

Chang Gung University

Research output: Contribution to journal › Journal Article › peer-review

27 Scopus citations

Abstract

The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics.

Original language	English
Pages (from-to)	396-403
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	333
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2005.04.176
State	Published - 29 07 2005

Keywords

Nucleophosmin/B23
Rev peptide
p53

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2005.04.176

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title = "Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53",

abstract = "The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics.",

keywords = "Nucleophosmin/B23, Rev peptide, p53",

author = "Chan, \{Hui Jia\} and Weng, \{Jing Jei\} and Yung, \{Benjamin Yat Ming\}",

year = "2005",

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day = "29",

doi = "10.1016/j.bbrc.2005.04.176",

language = "英语",

volume = "333",

pages = "396--403",

journal = "Biochemical and Biophysical Research Communications",

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}

TY - JOUR

T1 - Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53

AU - Chan, Hui Jia

AU - Weng, Jing Jei

AU - Yung, Benjamin Yat Ming

PY - 2005/7/29

Y1 - 2005/7/29

N2 - The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics.

AB - The Rev peptide that binds to nucleophosmin/B23 with the highest affinity exhibited the greatest cytotoxicity on Ras-3T3 cells and inhibited tumor growth most effectively in nude mice. The efficiency of colony formation in soft agar of Ras-3T3 cells was significantly inhibited by treatment with Rev peptide. In addition, Rev peptide could potentiate the doxorubicin-induced decrease of cellular viability in U1 bladder cancer cells and inhibition of tumor growth in nude mice. Treatment of Rev peptide increased protein expression and transcriptional activity of p53 and inhibited the nucleophosmin/B23-mediated PCNA promoter activation. Peptides having high affinity of binding to molecular targets such as nucleophosmin/B23 represent a potentially useful approach to anti-cancer biotherapeutics.

KW - Nucleophosmin/B23

KW - Rev peptide

KW - p53

UR - https://www.scopus.com/pages/publications/20544446874

U2 - 10.1016/j.bbrc.2005.04.176

DO - 10.1016/j.bbrc.2005.04.176

M3 - 文章

C2 - 15950182

AN - SCOPUS:20544446874

SN - 0006-291X

VL - 333

SP - 396

EP - 403

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Nucleophosmin/B23-binding peptide inhibits tumor growth and up-regulates transcriptional activity of p53

Abstract

Keywords

UN SDGs

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