One-pot fabrication of sacchachitin for production of TEMPO-oxidized sacchachitin nanofibers (TOSCNFs) utilized as scaffolds to enhance bone regeneration

Meng Huang Wu; Weu Wang; Fang Ching Chao; Chien Ming Hsieh; Ling Chun Chen; Hong Liang Lin; Hsiu O. Ho; Tsung Jen Huang; Ming Thau Sheu

doi:10.1016/j.carbpol.2020.117270

One-pot fabrication of sacchachitin for production of TEMPO-oxidized sacchachitin nanofibers (TOSCNFs) utilized as scaffolds to enhance bone regeneration

Meng Huang Wu, Weu Wang, Fang Ching Chao, Chien Ming Hsieh, Ling Chun Chen, Hong Liang Lin, Hsiu O. Ho, Tsung Jen Huang^*, Ming Thau Sheu

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

6 Scopus citations

Abstract

One-pot fabrication of sacchachitin (SC) for mass-production was developed and optimized by selecting KOH as alkaline agent in depigmentation step and utilizing NaClO₂ as bleaching agent in subsequent step in the same pot. Overall yield of one-pot-fabricated SC was up to 35 %w/w of initial weight with a fibrous texture soft enough for mechanical disintegration into SC nanofibers (SCNFs) and better dispersion for producing TEMPO-oxidized SCNFs (T033SC). Both SCNFs and T033SC could form a 3D gelatinous scaffold into which MC3T3-E1 cells were attracted. Higher calcium-trapping ability of T033SC resulting from a greater extent of carboxylate groups provided an excellent bone regeneration environment that resulted in better outcomes of bone regeneration in a femur defect rat model compared to those with SCNFs possessed fewer carboxylate groups. In conclusion, biomaterial scaffolds based on TEMPO-oxidized SCNFs produced from one-pot fabricated SC showed great potential for bone regeneration due to unique physical and chemical properties.

Original language	English
Article number	117270
Journal	Carbohydrate Polymers
Volume	254
DOIs	https://doi.org/10.1016/j.carbpol.2020.117270
State	Published - 15 02 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

2,2,6,6-Tetramethyl-1-piperidinyloxy (TEMPO)
Acetic acid
Bone regeneration
Citric acid
Ganoderma
Hydrogen peroxide solution (30 wt.% HO)
One-pot fabrication
Potassium hydroxide
Sacchachitin nanofibers
Sodium bromide
Sodium chlorite (NaClO)
Sodium hypochlorite
TEMPO-oxidation
Thiazolyl blue tetrazolium bromide (MTT reagent)

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10.1016/j.carbpol.2020.117270

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title = "One-pot fabrication of sacchachitin for production of TEMPO-oxidized sacchachitin nanofibers (TOSCNFs) utilized as scaffolds to enhance bone regeneration",

abstract = "One-pot fabrication of sacchachitin (SC) for mass-production was developed and optimized by selecting KOH as alkaline agent in depigmentation step and utilizing NaClO2 as bleaching agent in subsequent step in the same pot. Overall yield of one-pot-fabricated SC was up to 35 %w/w of initial weight with a fibrous texture soft enough for mechanical disintegration into SC nanofibers (SCNFs) and better dispersion for producing TEMPO-oxidized SCNFs (T033SC). Both SCNFs and T033SC could form a 3D gelatinous scaffold into which MC3T3-E1 cells were attracted. Higher calcium-trapping ability of T033SC resulting from a greater extent of carboxylate groups provided an excellent bone regeneration environment that resulted in better outcomes of bone regeneration in a femur defect rat model compared to those with SCNFs possessed fewer carboxylate groups. In conclusion, biomaterial scaffolds based on TEMPO-oxidized SCNFs produced from one-pot fabricated SC showed great potential for bone regeneration due to unique physical and chemical properties.",

keywords = "2,2,6,6-Tetramethyl-1-piperidinyloxy (TEMPO), Acetic acid, Bone regeneration, Citric acid, Ganoderma, Hydrogen peroxide solution (30 wt.% HO), One-pot fabrication, Potassium hydroxide, Sacchachitin nanofibers, Sodium bromide, Sodium chlorite (NaClO), Sodium hypochlorite, TEMPO-oxidation, Thiazolyl blue tetrazolium bromide (MTT reagent)",

author = "Wu, {Meng Huang} and Weu Wang and Chao, {Fang Ching} and Hsieh, {Chien Ming} and Chen, {Ling Chun} and Lin, {Hong Liang} and Ho, {Hsiu O.} and Huang, {Tsung Jen} and Sheu, {Ming Thau}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2021",

month = feb,

day = "15",

doi = "10.1016/j.carbpol.2020.117270",

language = "英语",

volume = "254",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - One-pot fabrication of sacchachitin for production of TEMPO-oxidized sacchachitin nanofibers (TOSCNFs) utilized as scaffolds to enhance bone regeneration

AU - Wu, Meng Huang

AU - Wang, Weu

AU - Chao, Fang Ching

AU - Hsieh, Chien Ming

AU - Chen, Ling Chun

AU - Lin, Hong Liang

AU - Ho, Hsiu O.

AU - Huang, Tsung Jen

AU - Sheu, Ming Thau

PY - 2021/2/15

Y1 - 2021/2/15

N2 - One-pot fabrication of sacchachitin (SC) for mass-production was developed and optimized by selecting KOH as alkaline agent in depigmentation step and utilizing NaClO2 as bleaching agent in subsequent step in the same pot. Overall yield of one-pot-fabricated SC was up to 35 %w/w of initial weight with a fibrous texture soft enough for mechanical disintegration into SC nanofibers (SCNFs) and better dispersion for producing TEMPO-oxidized SCNFs (T033SC). Both SCNFs and T033SC could form a 3D gelatinous scaffold into which MC3T3-E1 cells were attracted. Higher calcium-trapping ability of T033SC resulting from a greater extent of carboxylate groups provided an excellent bone regeneration environment that resulted in better outcomes of bone regeneration in a femur defect rat model compared to those with SCNFs possessed fewer carboxylate groups. In conclusion, biomaterial scaffolds based on TEMPO-oxidized SCNFs produced from one-pot fabricated SC showed great potential for bone regeneration due to unique physical and chemical properties.

AB - One-pot fabrication of sacchachitin (SC) for mass-production was developed and optimized by selecting KOH as alkaline agent in depigmentation step and utilizing NaClO2 as bleaching agent in subsequent step in the same pot. Overall yield of one-pot-fabricated SC was up to 35 %w/w of initial weight with a fibrous texture soft enough for mechanical disintegration into SC nanofibers (SCNFs) and better dispersion for producing TEMPO-oxidized SCNFs (T033SC). Both SCNFs and T033SC could form a 3D gelatinous scaffold into which MC3T3-E1 cells were attracted. Higher calcium-trapping ability of T033SC resulting from a greater extent of carboxylate groups provided an excellent bone regeneration environment that resulted in better outcomes of bone regeneration in a femur defect rat model compared to those with SCNFs possessed fewer carboxylate groups. In conclusion, biomaterial scaffolds based on TEMPO-oxidized SCNFs produced from one-pot fabricated SC showed great potential for bone regeneration due to unique physical and chemical properties.

KW - 2,2,6,6-Tetramethyl-1-piperidinyloxy (TEMPO)

KW - Acetic acid

KW - Bone regeneration

KW - Citric acid

KW - Ganoderma

KW - Hydrogen peroxide solution (30 wt.% HO)

KW - One-pot fabrication

KW - Potassium hydroxide

KW - Sacchachitin nanofibers

KW - Sodium bromide

KW - Sodium chlorite (NaClO)

KW - Sodium hypochlorite

KW - TEMPO-oxidation

KW - Thiazolyl blue tetrazolium bromide (MTT reagent)

UR - http://www.scopus.com/inward/record.url?scp=85096185384&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2020.117270

DO - 10.1016/j.carbpol.2020.117270

M3 - 文章

C2 - 33357851

AN - SCOPUS:85096185384

SN - 0144-8617

VL - 254

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

M1 - 117270

ER -

One-pot fabrication of sacchachitin for production of TEMPO-oxidized sacchachitin nanofibers (TOSCNFs) utilized as scaffolds to enhance bone regeneration

Abstract

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Keywords

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