TY - JOUR
T1 - Opening of the Blood-Brain Barrier by Low-Frequency (28-kHz) Ultrasound
T2 - A Novel Pinhole-Assisted Mechanical Scanning Device
AU - Liu, Hao Li
AU - Pan, Chia Hsin
AU - Ting, Cheng Yu
AU - Hsiao, Ming Jie
PY - 2010/2
Y1 - 2010/2
N2 - Disruption of the blood-brain barrier (BBB) may be transiently achieved via high-frequency focused spherical ultrasound in the presence of microbubbles. In this experimental animal study, we sought to determine whether focal reversible opening of the BBB may be achieved using low-frequency (i.e., 20-30 kHz) planar ultrasonic waves. In the presence of microbubbles, we were able to obtain BBB opening using non-focused ultrasound irradiation with a frequency as low as 28 kHz. We also achieved a tight regulation of the ultrasound patterns by using a mechanical scanning device equipped with a pinhole. Histologic examination of the brains supported the feasibility of our system. The areas of BBB disruption obtained with this method were large enough to cover a typical circumscribed cerebral tumor mass. The inherent advantages of our BBB opening method include an improved portability, the possibility to obtain fairly wide areas of BBB opening and a low incidence of hemorrhagic complications. In addition, our system has the potential to reduce the need for image guidance for treating superficial brain lesions. (E-mail: [email protected]).
AB - Disruption of the blood-brain barrier (BBB) may be transiently achieved via high-frequency focused spherical ultrasound in the presence of microbubbles. In this experimental animal study, we sought to determine whether focal reversible opening of the BBB may be achieved using low-frequency (i.e., 20-30 kHz) planar ultrasonic waves. In the presence of microbubbles, we were able to obtain BBB opening using non-focused ultrasound irradiation with a frequency as low as 28 kHz. We also achieved a tight regulation of the ultrasound patterns by using a mechanical scanning device equipped with a pinhole. Histologic examination of the brains supported the feasibility of our system. The areas of BBB disruption obtained with this method were large enough to cover a typical circumscribed cerebral tumor mass. The inherent advantages of our BBB opening method include an improved portability, the possibility to obtain fairly wide areas of BBB opening and a low incidence of hemorrhagic complications. In addition, our system has the potential to reduce the need for image guidance for treating superficial brain lesions. (E-mail: [email protected]).
KW - Blood-brain barrier disruption
KW - Low-frequency ultrasound
KW - Pinhole-assisted mechanical scanning
UR - http://www.scopus.com/inward/record.url?scp=74849090327&partnerID=8YFLogxK
U2 - 10.1016/j.ultrasmedbio.2009.10.004
DO - 10.1016/j.ultrasmedbio.2009.10.004
M3 - 文章
C2 - 20018435
AN - SCOPUS:74849090327
SN - 0301-5629
VL - 36
SP - 325
EP - 335
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 2
ER -