TY - JOUR
T1 - Optimal conditions for proliferation of bone marrow‐derived mouse macrophages in culture
T2 - The roles of CSF‐1, serum, Ca2+, and adherence
AU - Hume, David A.
AU - Gordon, Siamon
PY - 1983/11
Y1 - 1983/11
N2 - A method is described for the analysis of [3H]‐thymidine incorporation in microtitre cultures of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating factor (CSF‐1). [3H]‐thymidine incorporation depends on cell density, culture medium, and the concentration of CSF‐1 and serum, but is independent of Ca2+. Bone marrow‐derived macrophages are strongly adherent, but adherence can be dissociated from [3H]‐thymidine incorporation.
AB - A method is described for the analysis of [3H]‐thymidine incorporation in microtitre cultures of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating factor (CSF‐1). [3H]‐thymidine incorporation depends on cell density, culture medium, and the concentration of CSF‐1 and serum, but is independent of Ca2+. Bone marrow‐derived macrophages are strongly adherent, but adherence can be dissociated from [3H]‐thymidine incorporation.
UR - http://www.scopus.com/inward/record.url?scp=0021044136&partnerID=8YFLogxK
U2 - 10.1002/jcp.1041170209
DO - 10.1002/jcp.1041170209
M3 - 文章
C2 - 6605349
AN - SCOPUS:0021044136
SN - 0021-9541
VL - 117
SP - 189
EP - 194
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 2
ER -