Optimal conditions for proliferation of bone marrow‐derived mouse macrophages in culture: The roles of CSF‐1, serum, Ca2+, and adherence

David A. Hume; Siamon Gordon

doi:10.1002/jcp.1041170209

Optimal conditions for proliferation of bone marrow‐derived mouse macrophages in culture: The roles of CSF‐1, serum, Ca²⁺, and adherence

David A. Hume
, Siamon Gordon^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

82 Scopus citations

Abstract

A method is described for the analysis of [³H]‐thymidine incorporation in microtitre cultures of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating factor (CSF‐1). [³H]‐thymidine incorporation depends on cell density, culture medium, and the concentration of CSF‐1 and serum, but is independent of Ca²⁺. Bone marrow‐derived macrophages are strongly adherent, but adherence can be dissociated from [³H]‐thymidine incorporation.

Original language	English
Pages (from-to)	189-194
Number of pages	6
Journal	Journal of Cellular Physiology
Volume	117
Issue number	2
DOIs	https://doi.org/10.1002/jcp.1041170209
State	Published - 11 1983
Externally published	Yes

Access to Document

10.1002/jcp.1041170209

Cite this

@article{2819b06986334ad19645fa022cc93ed5,

title = "Optimal conditions for proliferation of bone marrow‐derived mouse macrophages in culture: The roles of CSF‐1, serum, Ca2+, and adherence",

abstract = "A method is described for the analysis of [3H]‐thymidine incorporation in microtitre cultures of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating factor (CSF‐1). [3H]‐thymidine incorporation depends on cell density, culture medium, and the concentration of CSF‐1 and serum, but is independent of Ca2+. Bone marrow‐derived macrophages are strongly adherent, but adherence can be dissociated from [3H]‐thymidine incorporation.",

author = "Hume, \{David A.\} and Siamon Gordon",

year = "1983",

month = nov,

doi = "10.1002/jcp.1041170209",

language = "英语",

volume = "117",

pages = "189--194",

journal = "Journal of Cellular Physiology",

issn = "0021-9541",

publisher = "Wiley-Liss Inc.",

number = "2",

}

TY - JOUR

T1 - Optimal conditions for proliferation of bone marrow‐derived mouse macrophages in culture

T2 - The roles of CSF‐1, serum, Ca2+, and adherence

AU - Hume, David A.

AU - Gordon, Siamon

PY - 1983/11

Y1 - 1983/11

N2 - A method is described for the analysis of [3H]‐thymidine incorporation in microtitre cultures of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating factor (CSF‐1). [3H]‐thymidine incorporation depends on cell density, culture medium, and the concentration of CSF‐1 and serum, but is independent of Ca2+. Bone marrow‐derived macrophages are strongly adherent, but adherence can be dissociated from [3H]‐thymidine incorporation.

AB - A method is described for the analysis of [3H]‐thymidine incorporation in microtitre cultures of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating factor (CSF‐1). [3H]‐thymidine incorporation depends on cell density, culture medium, and the concentration of CSF‐1 and serum, but is independent of Ca2+. Bone marrow‐derived macrophages are strongly adherent, but adherence can be dissociated from [3H]‐thymidine incorporation.

UR - https://www.scopus.com/pages/publications/0021044136

U2 - 10.1002/jcp.1041170209

DO - 10.1002/jcp.1041170209

M3 - 文章

C2 - 6605349

AN - SCOPUS:0021044136

SN - 0021-9541

VL - 117

SP - 189

EP - 194

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

IS - 2

ER -

Optimal conditions for proliferation of bone marrow‐derived mouse macrophages in culture: The roles of CSF‐1, serum, Ca²⁺, and adherence

Abstract

Access to Document

Other files and links

Fingerprint

Cite this