Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

Yung Luen Shih; Man Kuan Au; Ko Lin Liu; Ming Yang Yeh; Ching Hsiao Lee; Mei Hui Lee; Hsu Feng Lu; Jiun Long Yang; Rick Sai Chuen Wu; Jing Gung Chung

doi:10.1002/tox.22453

Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

Yung Luen Shih, Man Kuan Au, Ko Lin Liu, Ming Yang Yeh, Ching Hsiao Lee, Mei Hui Lee, Hsu Feng Lu^*, Jiun Long Yang, Rick Sai Chuen Wu, Jing Gung Chung

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

19 Scopus citations

Abstract

Ouabain, the specific Na⁺/K⁺-ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.

Original language	English
Pages (from-to)	2400-2413
Number of pages	14
Journal	Environmental Toxicology
Volume	32
Issue number	11
DOIs	https://doi.org/10.1002/tox.22453
State	Published - 11 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Wiley Periodicals, Inc.

Keywords

2 and 3
MMP-2
TGFB1
U-2 OS cells
migration and invasion
ouabain

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/tox.22453

Cite this

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title = "Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells",

abstract = "Ouabain, the specific Na+/K+-ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.",

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author = "Shih, {Yung Luen} and Au, {Man Kuan} and Liu, {Ko Lin} and Yeh, {Ming Yang} and Lee, {Ching Hsiao} and Lee, {Mei Hui} and Lu, {Hsu Feng} and Yang, {Jiun Long} and Wu, {Rick Sai Chuen} and Chung, {Jing Gung}",

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doi = "10.1002/tox.22453",

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volume = "32",

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AU - Shih, Yung Luen

AU - Au, Man Kuan

AU - Liu, Ko Lin

AU - Yeh, Ming Yang

AU - Lee, Ching Hsiao

AU - Lee, Mei Hui

AU - Lu, Hsu Feng

AU - Yang, Jiun Long

AU - Wu, Rick Sai Chuen

AU - Chung, Jing Gung

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N2 - Ouabain, the specific Na+/K+-ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.

AB - Ouabain, the specific Na+/K+-ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.

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Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

Abstract

Bibliographical note

Keywords

UN SDGs

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