Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

Yung Luen Shih; Man Kuan Au; Ko Lin Liu; Ming Yang Yeh; Ching Hsiao Lee; Mei Hui Lee; Hsu Feng Lu; Jiun Long Yang; Rick Sai Chuen Wu; Jing Gung Chung

doi:10.1002/tox.22453

Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

Yung Luen Shih
, Man Kuan Au
, Ko Lin Liu
, Ming Yang Yeh
, Ching Hsiao Lee
, Mei Hui Lee
, Hsu Feng Lu^*
, Jiun Long Yang
, Rick Sai Chuen Wu
, Jing Gung Chung

^*Corresponding author for this work

Shin Kong Wu Ho-Su Memorial Hospital
Taipei Medical University
Fu Jen Catholic University
Cheng Hsin General Hospital
Jen-Teh Junior College of Medicine, Nursing and Management
Changhua Christian Hospital
China Medical University Taichung
Asia University Taiwan

Research output: Contribution to journal › Journal Article › peer-review

21 Scopus citations

Abstract

Ouabain, the specific Na⁺/K⁺-ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.

Original language	English
Pages (from-to)	2400-2413
Number of pages	14
Journal	Environmental Toxicology
Volume	32
Issue number	11
DOIs	https://doi.org/10.1002/tox.22453
State	Published - 11 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Wiley Periodicals, Inc.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

2 and 3
MMP-2
TGFB1
U-2 OS cells
migration and invasion
ouabain

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10.1002/tox.22453

Cite this

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title = "Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells",

abstract = "Ouabain, the specific Na+/K+-ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.",

keywords = "2 and 3, MMP-2, TGFB1, U-2 OS cells, migration and invasion, ouabain",

author = "Shih, \{Yung Luen\} and Au, \{Man Kuan\} and Liu, \{Ko Lin\} and Yeh, \{Ming Yang\} and Lee, \{Ching Hsiao\} and Lee, \{Mei Hui\} and Lu, \{Hsu Feng\} and Yang, \{Jiun Long\} and Wu, \{Rick Sai Chuen\} and Chung, \{Jing Gung\}",

note = "Publisher Copyright: {\textcopyright} 2017 Wiley Periodicals, Inc.",

year = "2017",

month = nov,

doi = "10.1002/tox.22453",

language = "英语",

volume = "32",

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}

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T1 - Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

AU - Shih, Yung Luen

AU - Au, Man Kuan

AU - Liu, Ko Lin

AU - Yeh, Ming Yang

AU - Lee, Ching Hsiao

AU - Lee, Mei Hui

AU - Lu, Hsu Feng

AU - Yang, Jiun Long

AU - Wu, Rick Sai Chuen

AU - Chung, Jing Gung

PY - 2017/11

Y1 - 2017/11

N2 - Ouabain, the specific Na+/K+-ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.

AB - Ouabain, the specific Na+/K+-ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.

KW - 2 and 3

KW - MMP-2

KW - TGFB1

KW - U-2 OS cells

KW - migration and invasion

KW - ouabain

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ER -

Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

Abstract

Bibliographical note

UN SDGs

Keywords

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