Overexpression of a UV-damage recognition protein in a UV-sensitive human colon cancer cell line that features multidrug-resistant phenotype.

Chuck C.-K. Chao; NK Sun

doi:10.1006/bbrc.1993.1352

Overexpression of a UV-damage recognition protein in a UV-sensitive human colon cancer cell line that features multidrug-resistant phenotype.

Chuck C.-K. Chao, NK Sun

Department of Biochemistry

Research output: Contribution to journal › Journal Article › peer-review

Abstract

We have previously hypothesized that the level of UV-damage recognition protein (UVDRP) is a potential indicator of DNA-damage-mediated sensitivity or resistance of human cells to UV radiation [Chao, C.C.-K., Biochem. J. 282, 203-207, 1992]. In this study, we detected a 2-fold increase in the UVDRP binding activity in a UV-sensitive multidrug-resistant (MDR) human colon cancer cells compared to the parental SW620 cells. However, the MDR cells did not display enhanced DNA repair. The data is consistent with the conclusion that DNA excision repair is not the sole determinant of cellular sensitivity to genotoxic agents. The results also suggest that recognition of damaged DNA may be associated with generating a signal which mediates cellular sensitivity to UV radiation.

Original language	American English
Pages (from-to)	1252-1260
Journal	Biochemical and Biophysical Research Communications
Volume	191
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1993.1352
State	Published - 1993

Keywords

Cell Division/drug effects
Colonic Neoplasms/genetics
DNA Repair
DNA-Binding Proteins/metabolism
Drug Resistance
Gene Expression
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
RNA, Messenger/genetics
Tumor Cells, Cultured
Ultraviolet Rays

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/bbrc.1993.1352

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title = "Overexpression of a UV-damage recognition protein in a UV-sensitive human colon cancer cell line that features multidrug-resistant phenotype.",

abstract = "We have previously hypothesized that the level of UV-damage recognition protein (UVDRP) is a potential indicator of DNA-damage-mediated sensitivity or resistance of human cells to UV radiation [Chao, C.C.-K., Biochem. J. 282, 203-207, 1992]. In this study, we detected a 2-fold increase in the UVDRP binding activity in a UV-sensitive multidrug-resistant (MDR) human colon cancer cells compared to the parental SW620 cells. However, the MDR cells did not display enhanced DNA repair. The data is consistent with the conclusion that DNA excision repair is not the sole determinant of cellular sensitivity to genotoxic agents. The results also suggest that recognition of damaged DNA may be associated with generating a signal which mediates cellular sensitivity to UV radiation.",

keywords = "Cell Division/drug effects, Colonic Neoplasms/genetics, DNA Repair, DNA-Binding Proteins/metabolism, Drug Resistance, Gene Expression, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, RNA, Messenger/genetics, Tumor Cells, Cultured, Ultraviolet Rays",

author = "Chao, {Chuck C.-K.} and NK Sun",

year = "1993",

doi = "10.1006/bbrc.1993.1352",

language = "American English",

volume = "191",

pages = "1252--1260",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Overexpression of a UV-damage recognition protein in a UV-sensitive human colon cancer cell line that features multidrug-resistant phenotype.

AU - Chao, Chuck C.-K.

AU - Sun, NK

PY - 1993

Y1 - 1993

N2 - We have previously hypothesized that the level of UV-damage recognition protein (UVDRP) is a potential indicator of DNA-damage-mediated sensitivity or resistance of human cells to UV radiation [Chao, C.C.-K., Biochem. J. 282, 203-207, 1992]. In this study, we detected a 2-fold increase in the UVDRP binding activity in a UV-sensitive multidrug-resistant (MDR) human colon cancer cells compared to the parental SW620 cells. However, the MDR cells did not display enhanced DNA repair. The data is consistent with the conclusion that DNA excision repair is not the sole determinant of cellular sensitivity to genotoxic agents. The results also suggest that recognition of damaged DNA may be associated with generating a signal which mediates cellular sensitivity to UV radiation.

AB - We have previously hypothesized that the level of UV-damage recognition protein (UVDRP) is a potential indicator of DNA-damage-mediated sensitivity or resistance of human cells to UV radiation [Chao, C.C.-K., Biochem. J. 282, 203-207, 1992]. In this study, we detected a 2-fold increase in the UVDRP binding activity in a UV-sensitive multidrug-resistant (MDR) human colon cancer cells compared to the parental SW620 cells. However, the MDR cells did not display enhanced DNA repair. The data is consistent with the conclusion that DNA excision repair is not the sole determinant of cellular sensitivity to genotoxic agents. The results also suggest that recognition of damaged DNA may be associated with generating a signal which mediates cellular sensitivity to UV radiation.

KW - Cell Division/drug effects

KW - Colonic Neoplasms/genetics

KW - DNA Repair

KW - DNA-Binding Proteins/metabolism

KW - Drug Resistance

KW - Gene Expression

KW - Nuclear Proteins/genetics

KW - Nuclear Proteins/metabolism

KW - RNA, Messenger/genetics

KW - Tumor Cells, Cultured

KW - Ultraviolet Rays

U2 - 10.1006/bbrc.1993.1352

DO - 10.1006/bbrc.1993.1352

M3 - Journal Article

C2 - 8466502

SN - 0006-291X

VL - 191

SP - 1252

EP - 1260

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Overexpression of a UV-damage recognition protein in a UV-sensitive human colon cancer cell line that features multidrug-resistant phenotype.

Abstract

Keywords

UN SDGs

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