P53 and Sp1 cooperate to regulate the expression of epstein-barr viral Zta protein

Huey Huey Chua, Hsin Yi Chiu, Sue Jane Lin, Pei Lun Weng, Jiun Han Lin, Shao Wen Wu, Shu Chun Tsai, Ching Hwa Tsai*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

13 Scopus citations


Epstein-Barr virus (EBV) belongs to the gammaherpesvirus family. To produce infectious progeny, EBV reactivates from latency into the lytic cycle by expressing the determinative lytic transactivator, Zta. In the presence of histone deacetylase inhibitor (HDACi), p53 is a prerequisite for the initiation of the EBV lytic cycle by facilitating the expression of Zta. In this study, a serial mutational analysis of Zta promoter (Zp) indicated an important role for the ZID element in responding to HDACi induction and p53 binds to this ZID element together with Sp1, a universal transcription factor. Abolition of the DNA-binding ability of Sp1 reduces the inducibility of ZID by HDACi and also reduces the amount of p53 binding to ZID. Finally, it was shown that EBV in p53-positive-lymphoblastoid cell lines (LCLs) can enter into the lytic cycle spontaneously; however, knockdown of p53 in LCLs leads to retardation of EBV reactivation.

Original languageEnglish
Pages (from-to)1279-1288
Number of pages10
JournalJournal of Medical Virology
Issue number8
StatePublished - 08 2012
Externally publishedYes


  • EBV
  • P53
  • Reactivation
  • Sp1
  • Zta


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