TY - JOUR
T1 - Palladium-platinum bimetallic nanomaterials and their application in Staphylococcus aureus detection on paper-based devices
AU - Wu, Shou Cheng
AU - Tsai, Tsung Ting
AU - Li, Tsung Hua
AU - Tung, Cheng Yang
AU - Chiu, Ping Yeh
AU - Lin, Jia Hui
AU - Chen, Chien Fu
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/11/15
Y1 - 2022/11/15
N2 - In this study, we demonstrate how palladium@platinum nanoparticles (Pd@Pt NPs) can be used as a nanozyme for the detection of Staphylococcus aureus (S. aureus) on a paper-based analytical device. Pt was doped with Pd to form bimetallic NPs that serve as a peroxidase mimetic, which can enhance the reaction area with a substrate (3, 3', 5, 5'-tetramethylbenzidine, TMB). After material characterization, we show the peroxidase-like activity of the Pd@Pt NPs featured a 13-times higher binding affinity value (Km) for TMB compared to horseradish peroxidase, which is currently widely used in immunoassays. By incorporating the Pd@Pt NPs in a paper-based immunoassay, we can detect protein A, the biomarker of S. aureus, within 30 min with a detection limit of 9.56 ng/mL. We have also successfully validated this nanozyme-immobilized paper-based analytical device (nPAD) for the detection of human immunoglobulin G to demonstrate the capability of the Pd@Pt NPs for different target analytes. This highly sensitive, rapid, and portable nPAD design has the potential for personalized medicine and point-of-care testing, which could expand on-site prognoses in resource-limited settings.
AB - In this study, we demonstrate how palladium@platinum nanoparticles (Pd@Pt NPs) can be used as a nanozyme for the detection of Staphylococcus aureus (S. aureus) on a paper-based analytical device. Pt was doped with Pd to form bimetallic NPs that serve as a peroxidase mimetic, which can enhance the reaction area with a substrate (3, 3', 5, 5'-tetramethylbenzidine, TMB). After material characterization, we show the peroxidase-like activity of the Pd@Pt NPs featured a 13-times higher binding affinity value (Km) for TMB compared to horseradish peroxidase, which is currently widely used in immunoassays. By incorporating the Pd@Pt NPs in a paper-based immunoassay, we can detect protein A, the biomarker of S. aureus, within 30 min with a detection limit of 9.56 ng/mL. We have also successfully validated this nanozyme-immobilized paper-based analytical device (nPAD) for the detection of human immunoglobulin G to demonstrate the capability of the Pd@Pt NPs for different target analytes. This highly sensitive, rapid, and portable nPAD design has the potential for personalized medicine and point-of-care testing, which could expand on-site prognoses in resource-limited settings.
KW - Paper-based analytical device
KW - Pd@Pt nanoparticle
KW - Point-of-care testing
KW - Protein A
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=85137662395&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2022.114669
DO - 10.1016/j.bios.2022.114669
M3 - 文章
C2 - 36108567
AN - SCOPUS:85137662395
SN - 0956-5663
VL - 216
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 114669
ER -
