Partitioning of acid proteases in aqueous two-phase systems containing a charged polymer

Jyh Ping Chen

doi:10.1007/BF01875566

Partitioning of acid proteases in aqueous two-phase systems containing a charged polymer

Jyh Ping Chen^*

^*Corresponding author for this work

University of Wisconsin-Madison

Research output: Contribution to journal › Journal Article › peer-review

2 Scopus citations

Abstract

A preparation of DEAE-dextran was described, which can form an aqueous two-phase system with hydroxypropyldextran. Acid proteases showed strong partition in this system due to its large interfacial electrical potential. Partition coefficients of the enzymes changed rapidly when salts were added.

Original language	English
Pages (from-to)	131-134
Number of pages	4
Journal	Biotechnology Techniques
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/BF01875566
State	Published - 03 1989
Externally published	Yes

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title = "Partitioning of acid proteases in aqueous two-phase systems containing a charged polymer",

abstract = "A preparation of DEAE-dextran was described, which can form an aqueous two-phase system with hydroxypropyldextran. Acid proteases showed strong partition in this system due to its large interfacial electrical potential. Partition coefficients of the enzymes changed rapidly when salts were added.",

author = "Chen, \{Jyh Ping\}",

year = "1989",

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AB - A preparation of DEAE-dextran was described, which can form an aqueous two-phase system with hydroxypropyldextran. Acid proteases showed strong partition in this system due to its large interfacial electrical potential. Partition coefficients of the enzymes changed rapidly when salts were added.

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DO - 10.1007/BF01875566

M3 - 文章

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VL - 3

SP - 131

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JO - Biotechnology Techniques

JF - Biotechnology Techniques

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Partitioning of acid proteases in aqueous two-phase systems containing a charged polymer

Abstract

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