Passive biophysical membrane properties of nucleus reticularis gigantocellularis neurons in brain slices from the rat

Jiin Cherng Yen, Samuel H.H. Chan*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

3 Scopus citations

Abstract

This study employed intracellular recording coupled with the current clamp technique to characterize the passive biophysical membrane properties of neurons in the nucleus reticularis gigantocellularis (NRGC) of brain slices from adult, male Sprague-Dawley rats. We found that NRGC neurons possessed highly depolarized transmembrane potentials (-22.9 ± 0.6 mV, n = 189). The 29 NRGC neurons that received further evaluation showed that they could be separated into two clusters, with significantly different membrane input resistances (3.51 ± 0.89 vs 77.50 ± 8.82 M Ω) and membrane time constants (0.56 ± 0.05 vs 1.27 ± 0.12 ms). These membrane properties, which resembled qualitatively those we observed previously in cats in vivo, will form the basis for further evaluation of the ionic mechanisms that may underlie the actions of pharmacologic agents on the NRGC neurons.

Original languageEnglish
Pages (from-to)5-8
Number of pages4
JournalNeuroscience Letters
Volume159
Issue number1-2
DOIs
StatePublished - 03 09 1993
Externally publishedYes

Keywords

  • Brain slice
  • Membrane input resistance
  • Membrane time constant
  • Nucleus reticularis gigantocellularis
  • Rat
  • Transmembrane potential

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