Peripheral blood-derived endothelial progenitor cell therapy prevented deterioration of chronic kidney disease in rats

Tien Hung Huang; Yen Ta Chen; Pei Hsun Sung; Hsin Ju Chiang; Yung Lung Chen; Han Tan Chai; Sheng Ying Chung; Tzu Hsien Tsai; Chih Chao Yang; Chih Hung Chen; Yi Ling Chen; Hsueh Wen Chang; Cheuk Kwan Sun; Hon Kan Yip

Peripheral blood-derived endothelial progenitor cell therapy prevented deterioration of chronic kidney disease in rats

Tien Hung Huang, Yen Ta Chen, Pei Hsun Sung, Hsin Ju Chiang, Yung Lung Chen, Han Tan Chai, Sheng Ying Chung, Tzu Hsien Tsai, Chih Chao Yang, Chih Hung Chen, Yi Ling Chen, Hsueh Wen Chang, Cheuk Kwan Sun, Hon Kan Yip^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

39 Scopus citations

Abstract

Background: This study tested the hypothesis that peripheral blood-derived endothelial progenitor cell (PBDEPC) therapy can impede the deterioration of chronic kidney disease (CKD) induced by 5/6 nephrectomy in rats. Methods and results: Adult-male rats (n = 30) were equally categorized into group 1 (sham control), group 2 (CKD only) and group 3 [CKD + PBDEPC (left intra-arterial (3.3 × 10⁵) and penile vein (6.7 × 10⁵) injections by day 14 after CKD induction]. By day 60, kidney blood fow (KBF) was significantly lower in group 2 than that in groups 1 and 3, and significantly lower in group 3 than that in group 1, whereas the levels of serum creatinine, and kidney injury score and size showed an opposite pattern compared to that of KBF among all groups (all p < 0.001). Protein expressions of apoptotic (caspase 3, PARP), infammatory (TNF-α, MMP-9), oxidative-stress (oxidized protein, NOX-1), fbrotic (Smad3, TGF-β), and hypoxic/ischemic cell-stress (HIF-1α, p-Akt) biomarkers showed an opposite pattern, whereas angiogenesis at protein (eNOS, CD31) and cellular (CD31+, CXCR4+) levels showed an identical pattern compared to that of blood fow in all groups (all p < 0.01). Other pro-angiogenic biomarkers (SDF-1α, CXCR4, VEGF) at protein and cellular levels and antioxidants (HO-1+, NQO 1, GR+) at cellular level showed progressive significant increase from groups 1 to 3 (all p < 0.001). Conclusion: The results support that PBDEPC therapy effectively inhibits the propagation of CKD and the deterioration of renal function through enhancement of angiogenesis, blood fow, and anti-oxidative capacity as well as suppression of infammation, oxidative stress, apoptosis, and fbrosis in a rodent model.

Original language	English
Pages (from-to)	804-824
Number of pages	21
Journal	American Journal of Translational Research
Volume	7
Issue number	5
State	Published - 07 07 2015

Bibliographical note

Publisher Copyright:
© 2015, E-Century Publishing Corporation. All Rights Reserved.

Keywords

5/6 nephrectomy
Angiogenesis
Apoptosis
Chronic kidney disease
Infammation
Oxidative stress

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Huang, T. H., Chen, Y. T., Sung, P. H., Chiang, H. J., Chen, Y. L., Chai, H. T., Chung, S. Y., Tsai, T. H., Yang, C. C., Chen, C. H., Chen, Y. L., Chang, H. W., Sun, C. K., & Yip, H. K. (2015). Peripheral blood-derived endothelial progenitor cell therapy prevented deterioration of chronic kidney disease in rats. American Journal of Translational Research, 7(5), 804-824.

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title = "Peripheral blood-derived endothelial progenitor cell therapy prevented deterioration of chronic kidney disease in rats",

abstract = "Background: This study tested the hypothesis that peripheral blood-derived endothelial progenitor cell (PBDEPC) therapy can impede the deterioration of chronic kidney disease (CKD) induced by 5/6 nephrectomy in rats. Methods and results: Adult-male rats (n = 30) were equally categorized into group 1 (sham control), group 2 (CKD only) and group 3 [CKD + PBDEPC (left intra-arterial (3.3 × 105) and penile vein (6.7 × 105) injections by day 14 after CKD induction]. By day 60, kidney blood fow (KBF) was significantly lower in group 2 than that in groups 1 and 3, and significantly lower in group 3 than that in group 1, whereas the levels of serum creatinine, and kidney injury score and size showed an opposite pattern compared to that of KBF among all groups (all p < 0.001). Protein expressions of apoptotic (caspase 3, PARP), infammatory (TNF-α, MMP-9), oxidative-stress (oxidized protein, NOX-1), fbrotic (Smad3, TGF-β), and hypoxic/ischemic cell-stress (HIF-1α, p-Akt) biomarkers showed an opposite pattern, whereas angiogenesis at protein (eNOS, CD31) and cellular (CD31+, CXCR4+) levels showed an identical pattern compared to that of blood fow in all groups (all p < 0.01). Other pro-angiogenic biomarkers (SDF-1α, CXCR4, VEGF) at protein and cellular levels and antioxidants (HO-1+, NQO 1, GR+) at cellular level showed progressive significant increase from groups 1 to 3 (all p < 0.001). Conclusion: The results support that PBDEPC therapy effectively inhibits the propagation of CKD and the deterioration of renal function through enhancement of angiogenesis, blood fow, and anti-oxidative capacity as well as suppression of infammation, oxidative stress, apoptosis, and fbrosis in a rodent model.",

keywords = "5/6 nephrectomy, Angiogenesis, Apoptosis, Chronic kidney disease, Infammation, Oxidative stress",

author = "Huang, \{Tien Hung\} and Chen, \{Yen Ta\} and Sung, \{Pei Hsun\} and Chiang, \{Hsin Ju\} and Chen, \{Yung Lung\} and Chai, \{Han Tan\} and Chung, \{Sheng Ying\} and Tsai, \{Tzu Hsien\} and Yang, \{Chih Chao\} and Chen, \{Chih Hung\} and Chen, \{Yi Ling\} and Chang, \{Hsueh Wen\} and Sun, \{Cheuk Kwan\} and Yip, \{Hon Kan\}",

year = "2015",

month = jul,

day = "7",

language = "英语",

volume = "7",

pages = "804--824",

journal = "American Journal of Translational Research",

issn = "1943-8141",

publisher = "E-Century Publishing Corporation",

number = "5",

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TY - JOUR

T1 - Peripheral blood-derived endothelial progenitor cell therapy prevented deterioration of chronic kidney disease in rats

AU - Huang, Tien Hung

AU - Chen, Yen Ta

AU - Sung, Pei Hsun

AU - Chiang, Hsin Ju

AU - Chen, Yung Lung

AU - Chai, Han Tan

AU - Chung, Sheng Ying

AU - Tsai, Tzu Hsien

AU - Yang, Chih Chao

AU - Chen, Chih Hung

AU - Chen, Yi Ling

AU - Chang, Hsueh Wen

AU - Sun, Cheuk Kwan

AU - Yip, Hon Kan

PY - 2015/7/7

Y1 - 2015/7/7

N2 - Background: This study tested the hypothesis that peripheral blood-derived endothelial progenitor cell (PBDEPC) therapy can impede the deterioration of chronic kidney disease (CKD) induced by 5/6 nephrectomy in rats. Methods and results: Adult-male rats (n = 30) were equally categorized into group 1 (sham control), group 2 (CKD only) and group 3 [CKD + PBDEPC (left intra-arterial (3.3 × 105) and penile vein (6.7 × 105) injections by day 14 after CKD induction]. By day 60, kidney blood fow (KBF) was significantly lower in group 2 than that in groups 1 and 3, and significantly lower in group 3 than that in group 1, whereas the levels of serum creatinine, and kidney injury score and size showed an opposite pattern compared to that of KBF among all groups (all p < 0.001). Protein expressions of apoptotic (caspase 3, PARP), infammatory (TNF-α, MMP-9), oxidative-stress (oxidized protein, NOX-1), fbrotic (Smad3, TGF-β), and hypoxic/ischemic cell-stress (HIF-1α, p-Akt) biomarkers showed an opposite pattern, whereas angiogenesis at protein (eNOS, CD31) and cellular (CD31+, CXCR4+) levels showed an identical pattern compared to that of blood fow in all groups (all p < 0.01). Other pro-angiogenic biomarkers (SDF-1α, CXCR4, VEGF) at protein and cellular levels and antioxidants (HO-1+, NQO 1, GR+) at cellular level showed progressive significant increase from groups 1 to 3 (all p < 0.001). Conclusion: The results support that PBDEPC therapy effectively inhibits the propagation of CKD and the deterioration of renal function through enhancement of angiogenesis, blood fow, and anti-oxidative capacity as well as suppression of infammation, oxidative stress, apoptosis, and fbrosis in a rodent model.

AB - Background: This study tested the hypothesis that peripheral blood-derived endothelial progenitor cell (PBDEPC) therapy can impede the deterioration of chronic kidney disease (CKD) induced by 5/6 nephrectomy in rats. Methods and results: Adult-male rats (n = 30) were equally categorized into group 1 (sham control), group 2 (CKD only) and group 3 [CKD + PBDEPC (left intra-arterial (3.3 × 105) and penile vein (6.7 × 105) injections by day 14 after CKD induction]. By day 60, kidney blood fow (KBF) was significantly lower in group 2 than that in groups 1 and 3, and significantly lower in group 3 than that in group 1, whereas the levels of serum creatinine, and kidney injury score and size showed an opposite pattern compared to that of KBF among all groups (all p < 0.001). Protein expressions of apoptotic (caspase 3, PARP), infammatory (TNF-α, MMP-9), oxidative-stress (oxidized protein, NOX-1), fbrotic (Smad3, TGF-β), and hypoxic/ischemic cell-stress (HIF-1α, p-Akt) biomarkers showed an opposite pattern, whereas angiogenesis at protein (eNOS, CD31) and cellular (CD31+, CXCR4+) levels showed an identical pattern compared to that of blood fow in all groups (all p < 0.01). Other pro-angiogenic biomarkers (SDF-1α, CXCR4, VEGF) at protein and cellular levels and antioxidants (HO-1+, NQO 1, GR+) at cellular level showed progressive significant increase from groups 1 to 3 (all p < 0.001). Conclusion: The results support that PBDEPC therapy effectively inhibits the propagation of CKD and the deterioration of renal function through enhancement of angiogenesis, blood fow, and anti-oxidative capacity as well as suppression of infammation, oxidative stress, apoptosis, and fbrosis in a rodent model.

KW - 5/6 nephrectomy

KW - Angiogenesis

KW - Apoptosis

KW - Chronic kidney disease

KW - Infammation

KW - Oxidative stress

UR - https://www.scopus.com/pages/publications/84936758606

M3 - 文章

AN - SCOPUS:84936758606

SN - 1943-8141

VL - 7

SP - 804

EP - 824

JO - American Journal of Translational Research

JF - American Journal of Translational Research

IS - 5

ER -

Peripheral blood-derived endothelial progenitor cell therapy prevented deterioration of chronic kidney disease in rats

Abstract

Bibliographical note

Keywords

UN SDGs

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