pH-Responsive Triplex DNA Nanoswitches: Surface Plasmon Resonance Platform for Bladder Cancer-Associated microRNAs

Pei Ying Lin; Ying Feng Chang; Cheng Che Chen; Li Chen Su; Itamar Willner; Ja An Annie Ho

doi:10.1021/acsnano.4c16396

pH-Responsive Triplex DNA Nanoswitches: Surface Plasmon Resonance Platform for Bladder Cancer-Associated microRNAs

Pei Ying Lin, Ying Feng Chang, Cheng Che Chen, Li Chen Su, Itamar Willner^*, Ja An Annie Ho^*

^*Corresponding author for this work

Artificial Intelligence Research Center

Research output: Contribution to journal › Journal Article › peer-review

Abstract

Bladder cancer (BC) has a high recurrence rate, necessitating frequent monitoring. We herein present an innovative method for detecting BC-related miR-183 and miR-155 microRNAs using pH-responsive triplex DNA nanoswitches (TDNs). This approach employs a stepwise surface plasmon resonance biosensing platform (TDNs-SPR assay) to detect these two miRNAs sequentially. The platform involves the assembly of two triplex pH-responsive probes, switch A (SA) and switch B (SB), on an SPR sensing interface by anchoring the probes to the surface through SA/miR-183 and SB/miR-155 binding to the S9.6 antibody-modified surface. The probes are functionalized with streptavidin-Au nanoparticles/biotinylated strands, which act as reporter units for the presence of the respective miRNAs on the sensing interface. The pH-induced displacement of reporter units triggers stepwise SPR reflectivity changes: at pH 5.0 for sensing miR-183 and at pH 8.3 for sensing miR-155. The reflectivity changes relate quantitatively to the concentrations of miRNAs. This sensing platform enables the detection of two miRNAs with detection limits as low as 0.57 pM for miR-183 and 0.83 pM for miR-155, highlighting its powerful utility for precise biomarker analysis. Moreover, this platform distinguishes BC patients from healthy individuals in urine samples. The method offers a versatile, noninvasive method for detecting any two miRNAs associated with other diseases.

Original language	English
Pages (from-to)	7140-7153
Number of pages	14
Journal	ACS Nano
Volume	19
Issue number	7
DOIs	https://doi.org/10.1021/acsnano.4c16396
State	Published - 25 02 2025

Bibliographical note

Publisher Copyright:
© 2025 The Authors. Published by American Chemical Society.

Keywords

Au nanoparticle
DNA nanoswitch
antibody
biosensor
multiplex analysis
Surface Plasmon Resonance
Humans
Biosensing Techniques/methods
Metal Nanoparticles/chemistry
Gold/chemistry
Urinary Bladder Neoplasms/genetics
DNA/chemistry
MicroRNAs/urine
Hydrogen-Ion Concentration

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acsnano.4c16396

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@article{b9a88db88df94cb78e8a409ac9c805b0,

title = "pH-Responsive Triplex DNA Nanoswitches: Surface Plasmon Resonance Platform for Bladder Cancer-Associated microRNAs",

abstract = "Bladder cancer (BC) has a high recurrence rate, necessitating frequent monitoring. We herein present an innovative method for detecting BC-related miR-183 and miR-155 microRNAs using pH-responsive triplex DNA nanoswitches (TDNs). This approach employs a stepwise surface plasmon resonance biosensing platform (TDNs-SPR assay) to detect these two miRNAs sequentially. The platform involves the assembly of two triplex pH-responsive probes, switch A (SA) and switch B (SB), on an SPR sensing interface by anchoring the probes to the surface through SA/miR-183 and SB/miR-155 binding to the S9.6 antibody-modified surface. The probes are functionalized with streptavidin-Au nanoparticles/biotinylated strands, which act as reporter units for the presence of the respective miRNAs on the sensing interface. The pH-induced displacement of reporter units triggers stepwise SPR reflectivity changes: at pH 5.0 for sensing miR-183 and at pH 8.3 for sensing miR-155. The reflectivity changes relate quantitatively to the concentrations of miRNAs. This sensing platform enables the detection of two miRNAs with detection limits as low as 0.57 pM for miR-183 and 0.83 pM for miR-155, highlighting its powerful utility for precise biomarker analysis. Moreover, this platform distinguishes BC patients from healthy individuals in urine samples. The method offers a versatile, noninvasive method for detecting any two miRNAs associated with other diseases.",

keywords = "Au nanoparticle, DNA nanoswitch, antibody, biosensor, multiplex analysis, Surface Plasmon Resonance, Humans, Biosensing Techniques/methods, Metal Nanoparticles/chemistry, Gold/chemistry, Urinary Bladder Neoplasms/genetics, DNA/chemistry, MicroRNAs/urine, Hydrogen-Ion Concentration",

author = "Lin, {Pei Ying} and Chang, {Ying Feng} and Chen, {Cheng Che} and Su, {Li Chen} and Itamar Willner and Ho, {Ja An Annie}",

note = "Publisher Copyright: {\textcopyright} 2025 The Authors. Published by American Chemical Society.",

year = "2025",

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day = "25",

doi = "10.1021/acsnano.4c16396",

language = "英语",

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T1 - pH-Responsive Triplex DNA Nanoswitches

T2 - Surface Plasmon Resonance Platform for Bladder Cancer-Associated microRNAs

AU - Lin, Pei Ying

AU - Chang, Ying Feng

AU - Chen, Cheng Che

AU - Su, Li Chen

AU - Willner, Itamar

AU - Ho, Ja An Annie

PY - 2025/2/25

Y1 - 2025/2/25

N2 - Bladder cancer (BC) has a high recurrence rate, necessitating frequent monitoring. We herein present an innovative method for detecting BC-related miR-183 and miR-155 microRNAs using pH-responsive triplex DNA nanoswitches (TDNs). This approach employs a stepwise surface plasmon resonance biosensing platform (TDNs-SPR assay) to detect these two miRNAs sequentially. The platform involves the assembly of two triplex pH-responsive probes, switch A (SA) and switch B (SB), on an SPR sensing interface by anchoring the probes to the surface through SA/miR-183 and SB/miR-155 binding to the S9.6 antibody-modified surface. The probes are functionalized with streptavidin-Au nanoparticles/biotinylated strands, which act as reporter units for the presence of the respective miRNAs on the sensing interface. The pH-induced displacement of reporter units triggers stepwise SPR reflectivity changes: at pH 5.0 for sensing miR-183 and at pH 8.3 for sensing miR-155. The reflectivity changes relate quantitatively to the concentrations of miRNAs. This sensing platform enables the detection of two miRNAs with detection limits as low as 0.57 pM for miR-183 and 0.83 pM for miR-155, highlighting its powerful utility for precise biomarker analysis. Moreover, this platform distinguishes BC patients from healthy individuals in urine samples. The method offers a versatile, noninvasive method for detecting any two miRNAs associated with other diseases.

AB - Bladder cancer (BC) has a high recurrence rate, necessitating frequent monitoring. We herein present an innovative method for detecting BC-related miR-183 and miR-155 microRNAs using pH-responsive triplex DNA nanoswitches (TDNs). This approach employs a stepwise surface plasmon resonance biosensing platform (TDNs-SPR assay) to detect these two miRNAs sequentially. The platform involves the assembly of two triplex pH-responsive probes, switch A (SA) and switch B (SB), on an SPR sensing interface by anchoring the probes to the surface through SA/miR-183 and SB/miR-155 binding to the S9.6 antibody-modified surface. The probes are functionalized with streptavidin-Au nanoparticles/biotinylated strands, which act as reporter units for the presence of the respective miRNAs on the sensing interface. The pH-induced displacement of reporter units triggers stepwise SPR reflectivity changes: at pH 5.0 for sensing miR-183 and at pH 8.3 for sensing miR-155. The reflectivity changes relate quantitatively to the concentrations of miRNAs. This sensing platform enables the detection of two miRNAs with detection limits as low as 0.57 pM for miR-183 and 0.83 pM for miR-155, highlighting its powerful utility for precise biomarker analysis. Moreover, this platform distinguishes BC patients from healthy individuals in urine samples. The method offers a versatile, noninvasive method for detecting any two miRNAs associated with other diseases.

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KW - antibody

KW - biosensor

KW - multiplex analysis

KW - Surface Plasmon Resonance

KW - Humans

KW - Biosensing Techniques/methods

KW - Metal Nanoparticles/chemistry

KW - Gold/chemistry

KW - Urinary Bladder Neoplasms/genetics

KW - DNA/chemistry

KW - MicroRNAs/urine

KW - Hydrogen-Ion Concentration

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DO - 10.1021/acsnano.4c16396

M3 - 文章

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AN - SCOPUS:85217574812

SN - 1936-0851

VL - 19

SP - 7140

EP - 7153

JO - ACS Nano

JF - ACS Nano

IS - 7

ER -

pH-Responsive Triplex DNA Nanoswitches: Surface Plasmon Resonance Platform for Bladder Cancer-Associated microRNAs

Abstract

Bibliographical note

Keywords

UN SDGs

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