Phenotypic reversion of cisplatin resistance in human cells accompanies reduced host cell reactivation of damaged plasmid.

Chuck C.-K. Chao; YL Lee; S Lin-Chao

doi:10.1016/0006-291X(90)92169-Z

Phenotypic reversion of cisplatin resistance in human cells accompanies reduced host cell reactivation of damaged plasmid.

Chuck C.-K. Chao, YL Lee, S Lin-Chao

Department of Biochemistry

Research output: Contribution to journal › Journal Article › peer-review

Abstract

Revertant cell lines were established from cisplatin (CP) resistant HeLa cells. Expression of CP damaged plasmid DNA carrying bacterial chloramphenicol acetyltransferase (CAT) gene after transfection into cells was measured. Revertant cells showed reduced host cell reactivation of damaged plasmid, as compared to resistant cells. Addition of aphidicolin, an inhibitor for DNA polymerase alpha, to resistant cells effectively blocked enhanced plasmid reactivation and acquired resistance. The results are consistent with the notion that cellular ability in repair CP-damaged DNA is a mechanism for CP resistance.

Original language	American English
Pages (from-to)	851-859
Journal	Biochemical and Biophysical Research Communications
Volume	170
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(90)92169-Z
State	Published - 1990

Keywords

Aphidicolin
Cell Survival
Cisplatin/administration & dosage
Cisplatin/pharmacology
DNA Damage
DNA Repair
DNA/drug effects
Diterpenes/pharmacology
Dose-Response Relationship, Drug
Drug Resistance
HeLa Cells
Humans
Phenotype
Plasmids/drug effects

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10.1016/0006-291X(90)92169-Z

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@article{502a74510f2d442195153c0a78cfab34,

title = "Phenotypic reversion of cisplatin resistance in human cells accompanies reduced host cell reactivation of damaged plasmid.",

abstract = "Revertant cell lines were established from cisplatin (CP) resistant HeLa cells. Expression of CP damaged plasmid DNA carrying bacterial chloramphenicol acetyltransferase (CAT) gene after transfection into cells was measured. Revertant cells showed reduced host cell reactivation of damaged plasmid, as compared to resistant cells. Addition of aphidicolin, an inhibitor for DNA polymerase alpha, to resistant cells effectively blocked enhanced plasmid reactivation and acquired resistance. The results are consistent with the notion that cellular ability in repair CP-damaged DNA is a mechanism for CP resistance.",

keywords = "Aphidicolin, Cell Survival, Cisplatin/administration & dosage, Cisplatin/pharmacology, DNA Damage, DNA Repair, DNA/drug effects, Diterpenes/pharmacology, Dose-Response Relationship, Drug, Drug Resistance, HeLa Cells, Humans, Phenotype, Plasmids/drug effects",

author = "Chao, {Chuck C.-K.} and YL Lee and S Lin-Chao",

year = "1990",

doi = "10.1016/0006-291X(90)92169-Z",

language = "American English",

volume = "170",

pages = "851--859",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Phenotypic reversion of cisplatin resistance in human cells accompanies reduced host cell reactivation of damaged plasmid.

AU - Chao, Chuck C.-K.

AU - Lee, YL

AU - Lin-Chao, S

PY - 1990

Y1 - 1990

N2 - Revertant cell lines were established from cisplatin (CP) resistant HeLa cells. Expression of CP damaged plasmid DNA carrying bacterial chloramphenicol acetyltransferase (CAT) gene after transfection into cells was measured. Revertant cells showed reduced host cell reactivation of damaged plasmid, as compared to resistant cells. Addition of aphidicolin, an inhibitor for DNA polymerase alpha, to resistant cells effectively blocked enhanced plasmid reactivation and acquired resistance. The results are consistent with the notion that cellular ability in repair CP-damaged DNA is a mechanism for CP resistance.

AB - Revertant cell lines were established from cisplatin (CP) resistant HeLa cells. Expression of CP damaged plasmid DNA carrying bacterial chloramphenicol acetyltransferase (CAT) gene after transfection into cells was measured. Revertant cells showed reduced host cell reactivation of damaged plasmid, as compared to resistant cells. Addition of aphidicolin, an inhibitor for DNA polymerase alpha, to resistant cells effectively blocked enhanced plasmid reactivation and acquired resistance. The results are consistent with the notion that cellular ability in repair CP-damaged DNA is a mechanism for CP resistance.

KW - Aphidicolin

KW - Cell Survival

KW - Cisplatin/administration & dosage

KW - Cisplatin/pharmacology

KW - DNA Damage

KW - DNA Repair

KW - DNA/drug effects

KW - Diterpenes/pharmacology

KW - Dose-Response Relationship, Drug

KW - Drug Resistance

KW - HeLa Cells

KW - Humans

KW - Phenotype

KW - Plasmids/drug effects

U2 - 10.1016/0006-291X(90)92169-Z

DO - 10.1016/0006-291X(90)92169-Z

M3 - Journal Article

C2 - 2116798

SN - 0006-291X

VL - 170

SP - 851

EP - 859

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Phenotypic reversion of cisplatin resistance in human cells accompanies reduced host cell reactivation of damaged plasmid.

Abstract

Keywords

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