TY - JOUR
T1 - Phosphorylation activity associated with plasma membrane-cortical granule complex of xenopus laevis oocytes
AU - Young, George Pei Herng
AU - Koide, Samuel S.
AU - Young, John Ding E.
PY - 1988/6/30
Y1 - 1988/6/30
N2 - Plasma membrane-cortical granule complexes from Xenopus laevis oocytes were isolated by a differential centrifugation procedure. Membrane preparations incorporated 32P from [γ-32P]ATP predominantly into three protein bands of Mr 128,000, 54,000 and 29,000, as determined by sodium dodecyl-polyacrylamide gel electrophoresis. Progesterone and insulin, added in vitro to membranes, did not produce any change in this phosphorylation pattern, contrary to some previously published results. The availability of enriched membrane complexes should permit further biochemical dissection of signal transduction pathways that are activated during oocyte maturation and fertilization.
AB - Plasma membrane-cortical granule complexes from Xenopus laevis oocytes were isolated by a differential centrifugation procedure. Membrane preparations incorporated 32P from [γ-32P]ATP predominantly into three protein bands of Mr 128,000, 54,000 and 29,000, as determined by sodium dodecyl-polyacrylamide gel electrophoresis. Progesterone and insulin, added in vitro to membranes, did not produce any change in this phosphorylation pattern, contrary to some previously published results. The availability of enriched membrane complexes should permit further biochemical dissection of signal transduction pathways that are activated during oocyte maturation and fertilization.
UR - http://www.scopus.com/inward/record.url?scp=0024300124&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)81331-7
DO - 10.1016/S0006-291X(88)81331-7
M3 - 文章
C2 - 3291866
AN - SCOPUS:0024300124
SN - 0006-291X
VL - 153
SP - 1025
EP - 1031
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -