Phosphorylation activity associated with plasma membrane-cortical granule complex of xenopus laevis oocytes

George Pei Herng Young*, Samuel S. Koide, John Ding E. Young

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

2 Scopus citations

Abstract

Plasma membrane-cortical granule complexes from Xenopus laevis oocytes were isolated by a differential centrifugation procedure. Membrane preparations incorporated 32P from [γ-32P]ATP predominantly into three protein bands of Mr 128,000, 54,000 and 29,000, as determined by sodium dodecyl-polyacrylamide gel electrophoresis. Progesterone and insulin, added in vitro to membranes, did not produce any change in this phosphorylation pattern, contrary to some previously published results. The availability of enriched membrane complexes should permit further biochemical dissection of signal transduction pathways that are activated during oocyte maturation and fertilization.

Original languageEnglish
Pages (from-to)1025-1031
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume153
Issue number3
DOIs
StatePublished - 30 06 1988
Externally publishedYes

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