Photoluminescent graphene quantum dots for in vivo imaging of apoptotic cells

Prathik Roy; Arun Prakash Periasamy; Chiu Ya Lin; Guor Mour Her; Wei Jane Chiu; Chi Lin Li; Chia Lun Shu; Chih Ching Huang; Chi Te Liang; Huan Tsung Chang

doi:10.1039/c4nr07005d

Photoluminescent graphene quantum dots for in vivo imaging of apoptotic cells

Prathik Roy, Arun Prakash Periasamy, Chiu Ya Lin, Guor Mour Her, Wei Jane Chiu, Chi Lin Li, Chia Lun Shu, Chih Ching Huang^*, Chi Te Liang, Huan Tsung Chang

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

105 Scopus citations

Abstract

Apoptosis (programmed cell death) is linked to many incurable neurodegenerative, cardiovascular and cancer causing diseases. Numerous methods have been developed for imaging apoptotic cells in vitro; however, there are few methods available for imaging apoptotic cells in live animals (in vivo). Here we report a novel method utilizing the unique photoluminescence properties of plant leaf-derived graphene quantum dots (GQDs) modified with annexin V antibody (Ab_A5) to form (Ab_A5)-modified GQDs (Ab_A5-GQDs) enabling us to label apoptotic cells in live zebrafish (Danio rerio). The key is that zebrafish shows bright red photoluminescence in the presence of apoptotic cells. The toxicity of the GQDs has also been investigated with the GQDs exhibiting high biocompatibility as they were excreted from the zebrafish's body without affecting its growth significantly at a concentration lower than 2 mg mL^-1 over a period of 4 to 72 hour post fertilization. The GQDs have further been used to image human breast adenocarcinoma cell line (MCF-7 cells), human cervical cancer cell line (HeLa cells), and normal human mammary epithelial cell line (MCF-10A). These results are indispensable to further the advance of graphene-based nanomaterials for biomedical applications.

Original language	English
Pages (from-to)	2504-2510
Number of pages	7
Journal	Nanoscale
Volume	7
Issue number	6
DOIs	https://doi.org/10.1039/c4nr07005d
State	Published - 14 02 2015
Externally published	Yes

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Publisher Copyright:
© The Royal Society of Chemistry 2015.

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title = "Photoluminescent graphene quantum dots for in vivo imaging of apoptotic cells",

abstract = "Apoptosis (programmed cell death) is linked to many incurable neurodegenerative, cardiovascular and cancer causing diseases. Numerous methods have been developed for imaging apoptotic cells in vitro; however, there are few methods available for imaging apoptotic cells in live animals (in vivo). Here we report a novel method utilizing the unique photoluminescence properties of plant leaf-derived graphene quantum dots (GQDs) modified with annexin V antibody (AbA5) to form (AbA5)-modified GQDs (AbA5-GQDs) enabling us to label apoptotic cells in live zebrafish (Danio rerio). The key is that zebrafish shows bright red photoluminescence in the presence of apoptotic cells. The toxicity of the GQDs has also been investigated with the GQDs exhibiting high biocompatibility as they were excreted from the zebrafish's body without affecting its growth significantly at a concentration lower than 2 mg mL-1 over a period of 4 to 72 hour post fertilization. The GQDs have further been used to image human breast adenocarcinoma cell line (MCF-7 cells), human cervical cancer cell line (HeLa cells), and normal human mammary epithelial cell line (MCF-10A). These results are indispensable to further the advance of graphene-based nanomaterials for biomedical applications.",

author = "Prathik Roy and {Prakash Periasamy}, Arun and Lin, {Chiu Ya} and Her, {Guor Mour} and Chiu, {Wei Jane} and Li, {Chi Lin} and Shu, {Chia Lun} and Huang, {Chih Ching} and Liang, {Chi Te} and Chang, {Huan Tsung}",

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year = "2015",

month = feb,

day = "14",

doi = "10.1039/c4nr07005d",

language = "英语",

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AU - Roy, Prathik

AU - Prakash Periasamy, Arun

AU - Lin, Chiu Ya

AU - Her, Guor Mour

AU - Chiu, Wei Jane

AU - Li, Chi Lin

AU - Shu, Chia Lun

AU - Huang, Chih Ching

AU - Liang, Chi Te

AU - Chang, Huan Tsung

PY - 2015/2/14

Y1 - 2015/2/14

N2 - Apoptosis (programmed cell death) is linked to many incurable neurodegenerative, cardiovascular and cancer causing diseases. Numerous methods have been developed for imaging apoptotic cells in vitro; however, there are few methods available for imaging apoptotic cells in live animals (in vivo). Here we report a novel method utilizing the unique photoluminescence properties of plant leaf-derived graphene quantum dots (GQDs) modified with annexin V antibody (AbA5) to form (AbA5)-modified GQDs (AbA5-GQDs) enabling us to label apoptotic cells in live zebrafish (Danio rerio). The key is that zebrafish shows bright red photoluminescence in the presence of apoptotic cells. The toxicity of the GQDs has also been investigated with the GQDs exhibiting high biocompatibility as they were excreted from the zebrafish's body without affecting its growth significantly at a concentration lower than 2 mg mL-1 over a period of 4 to 72 hour post fertilization. The GQDs have further been used to image human breast adenocarcinoma cell line (MCF-7 cells), human cervical cancer cell line (HeLa cells), and normal human mammary epithelial cell line (MCF-10A). These results are indispensable to further the advance of graphene-based nanomaterials for biomedical applications.

AB - Apoptosis (programmed cell death) is linked to many incurable neurodegenerative, cardiovascular and cancer causing diseases. Numerous methods have been developed for imaging apoptotic cells in vitro; however, there are few methods available for imaging apoptotic cells in live animals (in vivo). Here we report a novel method utilizing the unique photoluminescence properties of plant leaf-derived graphene quantum dots (GQDs) modified with annexin V antibody (AbA5) to form (AbA5)-modified GQDs (AbA5-GQDs) enabling us to label apoptotic cells in live zebrafish (Danio rerio). The key is that zebrafish shows bright red photoluminescence in the presence of apoptotic cells. The toxicity of the GQDs has also been investigated with the GQDs exhibiting high biocompatibility as they were excreted from the zebrafish's body without affecting its growth significantly at a concentration lower than 2 mg mL-1 over a period of 4 to 72 hour post fertilization. The GQDs have further been used to image human breast adenocarcinoma cell line (MCF-7 cells), human cervical cancer cell line (HeLa cells), and normal human mammary epithelial cell line (MCF-10A). These results are indispensable to further the advance of graphene-based nanomaterials for biomedical applications.

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Photoluminescent graphene quantum dots for in vivo imaging of apoptotic cells

Abstract

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