TY - JOUR
T1 - Physical shish-kebab modification vs. chemical surface coating on expanded polytetrafluoroethylene vascular grafts for enhanced endothelial cell adhesion
AU - Wang, Dongfang
AU - Yu, Xueke
AU - Xu, Yiyang
AU - Wang, Xiaofeng
AU - Wang, Haonan
AU - Zhang, Yang
AU - Li, Qian
AU - Turng, Lih Sheng
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/8
Y1 - 2022/8
N2 - Promoting rapid adhesion of endothelial cells is of significance for the viability of vascular grafts for small-diameter blood vessels (SDBVs) made of expanded polytetrafluoroethylene (ePTFE). Many physical and chemical surface modification methods have been developed to overcome the inertness of ePTFE and to promote cell adhesion. In this study, a biomimetic poly(ε-caprolactone) (PCL) shish-kebab microstructure was formed on the fibrillated domains of ePTFE through induced crystallization. The surface morphology and chemistry, mechanical property, cytocompatibility, and endothelial cell adhesion of PCL shish-kebab modified ePTFE were investigated. The effectiveness of and synergy between this physical topology modification and the traditional chemical RGD dig-coating in terms of cell adhesion were also evaluated. The positive effect of the shish-kebab structure on cell adhesion was found to be more significant than that of RGD coating, and there is a strong synergy between ePTFE surface modification and RGD coating. The biomimetic shish-kebab structure could be a promising material platform for further grafting and/or surface functionalization of ePTFE for other tissue engineering applications.
AB - Promoting rapid adhesion of endothelial cells is of significance for the viability of vascular grafts for small-diameter blood vessels (SDBVs) made of expanded polytetrafluoroethylene (ePTFE). Many physical and chemical surface modification methods have been developed to overcome the inertness of ePTFE and to promote cell adhesion. In this study, a biomimetic poly(ε-caprolactone) (PCL) shish-kebab microstructure was formed on the fibrillated domains of ePTFE through induced crystallization. The surface morphology and chemistry, mechanical property, cytocompatibility, and endothelial cell adhesion of PCL shish-kebab modified ePTFE were investigated. The effectiveness of and synergy between this physical topology modification and the traditional chemical RGD dig-coating in terms of cell adhesion were also evaluated. The positive effect of the shish-kebab structure on cell adhesion was found to be more significant than that of RGD coating, and there is a strong synergy between ePTFE surface modification and RGD coating. The biomimetic shish-kebab structure could be a promising material platform for further grafting and/or surface functionalization of ePTFE for other tissue engineering applications.
KW - Endothelial cell adhesion
KW - Poly(ε-caprolactone) (PCL)
KW - Shish-kebab microstructure
KW - Surface functionalization
KW - Surface modification
KW - ePTFE vascular grafts
UR - http://www.scopus.com/inward/record.url?scp=85133250870&partnerID=8YFLogxK
U2 - 10.1016/j.matdes.2022.110889
DO - 10.1016/j.matdes.2022.110889
M3 - 文章
AN - SCOPUS:85133250870
SN - 0264-1275
VL - 220
JO - Materials and Design
JF - Materials and Design
M1 - 110889
ER -