Platelet glycoprotein IIb/IIIa Inhibitor Tirofiban Ameliorates Cardiac Reperfusion Injury

Shih Tai Chang*, Chang Min Chung, Chi Ming Chu, Teng Yao Yang, Kuo Li Pan, Jen Te Hsu, Ju Feng Hsiao

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

17 Scopus citations

Abstract

There are many published articles on the effects of the antithrombolytic function of platelet glycoprotein IIb/IIIa inhibitors (GP IIb/IIIa inhibitors) in myocardial infarction. However, few studies have explored the effects and optimal concentration of tirofibans in diminishing the extent of myocardial reperfusion injury (RI). Rats received 120 minutes of coronary ligation and 180 minutes of reperfusion. The rats were then divided into 7 groups based on the concentration of tirofiban administered intravenously 30 minutes prior to coronary reperfusion to the end of reperfusion. The ratio of myocardial necrotic area to area at risk (AAR), and myocardial malondialdehyde (MDA) and plasma myeloperoxidase (MPO) activities were measured. The apoptotic index (AI) was the percentage of myocytes positive for terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) out of all myocytes stained by 4’, 6-diamidino-2-phenylindole (DAPI). The ratio of myocardial necrotic area to AAR significantly decreased in all tirofiban subgroups. The MDA activity for tirofiban concentrations of 2 and 5 ug/kg/minute showed a slight reduction. MPO activity was significantly decreased at a tirofiban concentration of 2 ug/kg/minute. The AI was significantly decreased at a tirofiban concentration of ≥ 0.4 ug/kg/minute. The results indicate that a tirofiban can significantly ameliorate the cardiac RI and myocyte apoptosis in rats.

Original languageEnglish
Pages (from-to)335-340
Number of pages6
JournalInternational Heart Journal
Volume56
Issue number3
DOIs
StatePublished - 27 04 2015

Bibliographical note

Publisher Copyright:
© 2015, International Heart Journal Association. All rights reserves.

Keywords

  • Apoptosis
  • Coronary artery disease
  • IIb/IIIa platelet inhibitor

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