Polyvalency of Tn (GalNAcα1→Ser/Thr) glycotope as a critical factor for Vicia villosa B4 and glycoprotein interactions

Albert M. Wu

doi:10.1016/S0014-5793(04)00180-2

Polyvalency of Tn (GalNAcα1→Ser/Thr) glycotope as a critical factor for Vicia villosa B₄ and glycoprotein interactions

Albert M. Wu^*

^*Corresponding author for this work

Department of Molecular Biology

Research output: Contribution to journal › Journal Article › peer-review

38 Scopus citations

Abstract

Vicia villosa B₄ (VVL-B₄) is an important lectin for detecting exposed Tn (GalNAcα1-Ser/Thr) determinants on cancer cells. In order to elucidate the binding factors involved in VVL-B₄ and glycotope interaction, the binding properties of this lectin were analyzed by enzyme-linked lectinosorbent and inhibition assays. From the results, it is concluded that the most critical factor affecting VVL-B₄ binding is polyvalency at the α anomer of Gal with -NH CH₃CO at carbon-2 (Tn epitope), which enhances the reactivity by 3.3×10⁵ times over monovalent Gal. The reactivities of glycotopes can be ranked as follows: high density Tn cluster≫Tn glycopeptides (MW<3.0×10 ³)≫monomeric Tn to tri- Tn glycopeptides⋙other GalNAcα/β-related structural units>Gal and Galα- or β-linked ligands, demonstrating the essential role of the polyvalency of Tn glycotopes in the enhancement of the binding.

Original language	English
Pages (from-to)	51-58
Number of pages	8
Journal	FEBS Letters
Volume	562
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(04)00180-2
State	Published - 26 03 2004

Keywords

Carbohydrate specificity
D-Fuc, D-fucopyranose
GalNAc, 2-acetamido-2-deoxy-D-galactopyranose
Glc, D-glucopyranose
Glycoprotein binding
L-Ara, L-arabinose
Lectin
Multivalent effect
Polyvalency
Sugars: Gal, D-galactopyranose
Vicia villosa B

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0014-5793(04)00180-2

Cite this

@article{4ae2d49689c24db1a057c89551c54129,

title = "Polyvalency of Tn (GalNAcα1→Ser/Thr) glycotope as a critical factor for Vicia villosa B4 and glycoprotein interactions",

abstract = "Vicia villosa B4 (VVL-B4) is an important lectin for detecting exposed Tn (GalNAcα1-Ser/Thr) determinants on cancer cells. In order to elucidate the binding factors involved in VVL-B4 and glycotope interaction, the binding properties of this lectin were analyzed by enzyme-linked lectinosorbent and inhibition assays. From the results, it is concluded that the most critical factor affecting VVL-B4 binding is polyvalency at the α anomer of Gal with -NH CH3CO at carbon-2 (Tn epitope), which enhances the reactivity by 3.3×105 times over monovalent Gal. The reactivities of glycotopes can be ranked as follows: high density Tn cluster≫Tn glycopeptides (MW<3.0×10 3)≫monomeric Tn to tri- Tn glycopeptides⋙other GalNAcα/β-related structural units>Gal and Galα- or β-linked ligands, demonstrating the essential role of the polyvalency of Tn glycotopes in the enhancement of the binding.",

keywords = "Carbohydrate specificity, D-Fuc, D-fucopyranose, GalNAc, 2-acetamido-2-deoxy-D-galactopyranose, Glc, D-glucopyranose, Glycoprotein binding, L-Ara, L-arabinose, Lectin, Multivalent effect, Polyvalency, Sugars: Gal, D-galactopyranose, Vicia villosa B",

author = "Wu, \{Albert M.\}",

year = "2004",

month = mar,

day = "26",

doi = "10.1016/S0014-5793(04)00180-2",

language = "英语",

volume = "562",

pages = "51--58",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

number = "1-3",

}

TY - JOUR

T1 - Polyvalency of Tn (GalNAcα1→Ser/Thr) glycotope as a critical factor for Vicia villosa B4 and glycoprotein interactions

AU - Wu, Albert M.

PY - 2004/3/26

Y1 - 2004/3/26

N2 - Vicia villosa B4 (VVL-B4) is an important lectin for detecting exposed Tn (GalNAcα1-Ser/Thr) determinants on cancer cells. In order to elucidate the binding factors involved in VVL-B4 and glycotope interaction, the binding properties of this lectin were analyzed by enzyme-linked lectinosorbent and inhibition assays. From the results, it is concluded that the most critical factor affecting VVL-B4 binding is polyvalency at the α anomer of Gal with -NH CH3CO at carbon-2 (Tn epitope), which enhances the reactivity by 3.3×105 times over monovalent Gal. The reactivities of glycotopes can be ranked as follows: high density Tn cluster≫Tn glycopeptides (MW<3.0×10 3)≫monomeric Tn to tri- Tn glycopeptides⋙other GalNAcα/β-related structural units>Gal and Galα- or β-linked ligands, demonstrating the essential role of the polyvalency of Tn glycotopes in the enhancement of the binding.

AB - Vicia villosa B4 (VVL-B4) is an important lectin for detecting exposed Tn (GalNAcα1-Ser/Thr) determinants on cancer cells. In order to elucidate the binding factors involved in VVL-B4 and glycotope interaction, the binding properties of this lectin were analyzed by enzyme-linked lectinosorbent and inhibition assays. From the results, it is concluded that the most critical factor affecting VVL-B4 binding is polyvalency at the α anomer of Gal with -NH CH3CO at carbon-2 (Tn epitope), which enhances the reactivity by 3.3×105 times over monovalent Gal. The reactivities of glycotopes can be ranked as follows: high density Tn cluster≫Tn glycopeptides (MW<3.0×10 3)≫monomeric Tn to tri- Tn glycopeptides⋙other GalNAcα/β-related structural units>Gal and Galα- or β-linked ligands, demonstrating the essential role of the polyvalency of Tn glycotopes in the enhancement of the binding.

KW - Carbohydrate specificity

KW - D-Fuc, D-fucopyranose

KW - GalNAc, 2-acetamido-2-deoxy-D-galactopyranose

KW - Glc, D-glucopyranose

KW - Glycoprotein binding

KW - L-Ara, L-arabinose

KW - Lectin

KW - Multivalent effect

KW - Polyvalency

KW - Sugars: Gal, D-galactopyranose

KW - Vicia villosa B

UR - https://www.scopus.com/pages/publications/1642392364

U2 - 10.1016/S0014-5793(04)00180-2

DO - 10.1016/S0014-5793(04)00180-2

M3 - 文章

C2 - 15044001

AN - SCOPUS:1642392364

SN - 0014-5793

VL - 562

SP - 51

EP - 58

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -