Pore size and functional properties of defined MAC and poly C9 complexes: Reconstitution into model lipid membranes

John Ding E. Young; Zanvil A. Cohn; Eckhard R. Podack

doi:10.1201/9781351071307

Pore size and functional properties of defined MAC and poly C9 complexes: Reconstitution into model lipid membranes

John Ding E. Young, Zanvil A. Cohn, Eckhard R. Podack

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The membrane attack complex (MAC) of complement is formed by the assembly of the five terminal components of complement in the target cell membrane which results in membrane damage and cytolysis. Ultrastructural studies have shown that membranes damaged by the MAC show tubular membrane lesions of approximately 100 A in internal diameter.^1-4 These observations are consistent with the doughnut channel model for complement-mediated killing proposed earlier by Mayer. ⁵ The assembly of these membraneattack components involves an initial insertion of C5b-7 into membrane⁶, followed by the subsequent binding of C8 and C9 to form the tubular lesion^7,8. C9 appears to be responsible largely for the formation of the tubular structure associated with the MAC.^4,9,10 Polymerization of C9 into circular lesions occurs after prolonged incubation of the monomer at temperature exceeding 37°C.^11,12.

Original language	English
Title of host publication	Cytolytic Lymphocytes and Complement Effectors of the Immune System
Publisher	CRC Press
Pages	221-227
Number of pages	7
Volume	1
ISBN (Electronic)	9781351079754
ISBN (Print)	9781315892207
DOIs	https://doi.org/10.1201/9781351071307
State	Published - 01 01 2018

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Publisher Copyright:
© 1988 by CRC Press, Inc.

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abstract = "The membrane attack complex (MAC) of complement is formed by the assembly of the five terminal components of complement in the target cell membrane which results in membrane damage and cytolysis. Ultrastructural studies have shown that membranes damaged by the MAC show tubular membrane lesions of approximately 100 A in internal diameter.1-4 These observations are consistent with the doughnut channel model for complement-mediated killing proposed earlier by Mayer. 5 The assembly of these membraneattack components involves an initial insertion of C5b-7 into membrane6, followed by the subsequent binding of C8 and C9 to form the tubular lesion7,8. C9 appears to be responsible largely for the formation of the tubular structure associated with the MAC.4,9,10 Polymerization of C9 into circular lesions occurs after prolonged incubation of the monomer at temperature exceeding 37°C.11,12.",

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T2 - Reconstitution into model lipid membranes

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AU - Cohn, Zanvil A.

AU - Podack, Eckhard R.

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N2 - The membrane attack complex (MAC) of complement is formed by the assembly of the five terminal components of complement in the target cell membrane which results in membrane damage and cytolysis. Ultrastructural studies have shown that membranes damaged by the MAC show tubular membrane lesions of approximately 100 A in internal diameter.1-4 These observations are consistent with the doughnut channel model for complement-mediated killing proposed earlier by Mayer. 5 The assembly of these membraneattack components involves an initial insertion of C5b-7 into membrane6, followed by the subsequent binding of C8 and C9 to form the tubular lesion7,8. C9 appears to be responsible largely for the formation of the tubular structure associated with the MAC.4,9,10 Polymerization of C9 into circular lesions occurs after prolonged incubation of the monomer at temperature exceeding 37°C.11,12.

AB - The membrane attack complex (MAC) of complement is formed by the assembly of the five terminal components of complement in the target cell membrane which results in membrane damage and cytolysis. Ultrastructural studies have shown that membranes damaged by the MAC show tubular membrane lesions of approximately 100 A in internal diameter.1-4 These observations are consistent with the doughnut channel model for complement-mediated killing proposed earlier by Mayer. 5 The assembly of these membraneattack components involves an initial insertion of C5b-7 into membrane6, followed by the subsequent binding of C8 and C9 to form the tubular lesion7,8. C9 appears to be responsible largely for the formation of the tubular structure associated with the MAC.4,9,10 Polymerization of C9 into circular lesions occurs after prolonged incubation of the monomer at temperature exceeding 37°C.11,12.

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Pore size and functional properties of defined MAC and poly C9 complexes: Reconstitution into model lipid membranes

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